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Title
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Molecular modeling of DICER and identification of phosphorylation sites |
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Authors
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Priyanka Dhar*, Sayak Ganguli, Abhijit Datta | |
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Affiliation
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Defense Institute of High Altitude Research, Defence Research & Development Organization, C/o 56 APO, Pin- 901205, India
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| Fax | 01982-252096; *corresponding author | |
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Article Type
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Hypothesis | |
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Date
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Received October 29, 2009; accepted November 15, 2009; published March 31, 2010 | |
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Abstract |
RNA interference (RNAi) is a post-transcriptional process initiated by double-stranded RNA molecules that degrade a complementary target RNA. In the first step, long double-stranded RNA molecules are chopped into shorter duplexes by an endonuclease dubbed DICER. This work focused on the identification of phosphorylation sites in DICER and proceeded towards comparative modeling. The domain of unknown function in DICER showed homology with the chromatin remodeling protein domains. This suggests that DICER may also perform chromatin remodeling. The Ramachandran analysis revealed that the models had 96% residues in the permissible regions and could be used for future ligand binding studies.
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Keywords |
RNAi, DICER, protein phosphorylation, chromatin remodeling, conserved domain.
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Citation
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Dhar, Bioinformation 4(9): 412-416 (2010) | |
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Edited by
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P. Kangueane | |
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ISSN
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0973-2063
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Publisher
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License
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This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License. |