Title

PFA: Program for the Quantitative Assessment of Cell Metabolism by Spectral Data Analysis

Authors

Chris Orr^{1, *}, Stephen Scott¹, Fouad Kandeel¹ , Kevin Ferreri¹

Affiliation

¹Southern California Islet Cell Resources Center, Department of Diabetes, Endocrinology, and Metabolism, Beckman Research Institute of the City of Hope, Duarte, CA.

Email

rahsaan26@hotmail.com; * Corresponding author

Article Type

Software

Date

received September 02, 2008; accepted September 17, 2008; published October 18, 2008

Abstract

Assessment of mitochondrial oxidative metabolism has wide-ranging importance, from pharmacokinetic analysis to studies in cell viability and apoptosis. Here we present the Perfusion File Analyzer (PFA) application for the real-time analysis of spectral data to measure cytochrome c reduction, cytochrome a3 reduction, and other parameters important to cellular metabolism, which are collected during tissue perfusion experiments. Our current efforts are focused on quantitating changes in mitochondrial function by normalizing baseline drift of spectral data while addressing two major challenges: (1) a lack of real-time feedback from the system when aiming is compromised, and (2) an inability to adjust calculated data in the event of spectral shift. PFA has been developed to address these issues, and is currently used for quality assessment of human islets prior to clinical transplantation.

Keywords

 oxidative metabolism; cell; data analysis; pharmacokinetics analysis

Citation

Orr et al., Bioinformation 3(1): 65-67 (2008)

Edited by

P. Kangueane

ISSN

0973-2063

Publisher

Biomedical Informatics

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.