Title
Isolation and characterization of stem cells from human exfoliated deciduous teeth
Authors
Ratna Yumkham*,1, C. Nagarathna2, Nelson Sanjenbam3, Angom Gopilal Singh4, Heisnam Philip Singh5 & Albert Ashem6
Affiliation
1Department of Paediatric and Preventive Dentistry, Dental College, RIMS, Imphal, Manipur, India; 2Department of Pedodontics and Preventive Dentistry, RajaRajeswari Dental College and Hospital, Bangalore, Karnataka, India; 3Department of Oral and Maxillofacial Surgery Dental College, JNIMS, Porompat, Imphal, Manipur, India; 4Braces Dental Care and 3D Imaging Centre, Imphal, Manipur, India; 5Orthodontics and Dentofacial Orthopaedics Practioner, Imphal, Manipur, India; 6Department of Oral Medicine and radiology, Dental College, RIMS, Imphal, Manipur, India; *Corresponding author
Ratna Yumkham - E-mail: yumkhamratna@gmail.com; Phone: +91 9366377409
C. Nagarathna - E-mail: shaanrathna@gmail.com; Phone: +91 9845581779
Nelson Sanjenbam - E-mail: sanjenbamnelson@gmail.com; Phone: +91 9366585629
Angom Gopilal Singh - E-mail: bracesdentalcare2017@gmail.com; Phone: +91 9612651893
Heisnam philip Singh - E-mail: philips031@gmail.com; Phone: +91 8258948901
Albert Ashem - E-mail: albertashem@ymail.com; Phone: +91 9612284189
Article Type
Research Article
Date
Received May 1, 2024; Revised May 31, 2024; Accepted May 31, 2024, Published May 31, 2024
Abstract
SHEDs have been shown to have a higher rate of proliferation and raise in cell population doublings when compared to stem cells from permanent teeth. Hence, using them in tissue engineering may be advantageous over stem cells from adult human teeth. Stem cells were removed from pulpal tissues of thirty primary teeth undergoing extraction under six to fourteen year of age. The tissues were incubated after centrifuging and adding DMEM-KO following the addition of a 2 mg/ml collagenase blend for examination of plates in search of cell attachment and growth. Flow cytometric analysis showed successful isolation of SHEDs using fluoresce inisothiocyanate (FITC)-conjugated CD-34, CD-105, and PE (R-phycoerythrin)-conjugated CD-45, CD-90, CD-73, and HLA-DR antibodies. The surface antigens CD-73, CD-90 and CD-105 which are known to be present in mesenchymal lineages were positively expressed in SHEDs according to flow cytometry analysis, whereas CD-34, CD-45, and HLA-DR were not.
Keywords
Stem Cells; Multipotent Stem Cells; Dental pulp
Citation
Yumkham et al. Bioinformation 20(5): 557-561 (2024)
Edited by
P Kangueane
ISSN
0973-2063
Publisher
License
This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.
