Identification of Microbial Pathogens in Periodontal disease and Diabetic patients of South Indian Population

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Title	Identification of Microbial Pathogens in Periodontal disease and Diabetic patients of South Indian Population
Authors	Tikka Chiranjeevi¹, Osuru Hari Prasad¹, Uppu Venkateswara Prasad², Avula Kishor Kumar³, Veeraraghavulu Praveen Chakravarthi¹, Paramala Balaji Rao¹, Potuguchi Venkata Gurunadha Krishna Sarma², Nagi reddy Raveendra Reddy³, Matcha Bhaskar^1*
Affiliation	¹Division of Animal Biotechnology, Department of Zoology, S. V. University, Tirupati-517502, Andhra Pradesh, India; ²Department of Biotechnology, SVIMS, Tirupati-517502, Andhra Pradesh, India; ³Department of Periodontology, Krishna Teja Dental College, Tirupati, Andhra Pradesh, India
Email	matchabhaskar2010@gmail.com; *Corresponding authors
Article Type	Research note
Date	Received March 07, 2014; Accepted March 14, 2014; Published April 29, 2014
Abstract	Periodontitis have been referred to as the sixth complication of diabetes found in high prevalence among diabetic patients than among healthy controls. The aim of the present study was to examine the periodontal disease status among collected dental plaque samples. Chromosomal DNA was isolated and amplified by universal primers. The DNA was sequenced for bacterial confirmation and phylogenetic analysis performed for the evolutionary relationship with other known pathogens. No amplification products were observed in groups labeled non periodontal and non Diabetes (NP&ND) and non Periodontal and Diabetes (NP&D). But in the case of Periodontal and non Diabetes (P&ND) groups 22 amplified products were observed. In case of Periodontal and Diabetes (P&D), 32 amplified products were positive for microbes. Among the four microbial groups, Treponemadenticola, and Tannerella forsythia were found to be prevalent in P&D. The phylogenetic analysis of 16s rRNA of Treponemadenticola showed the relationship with other Treponema oral pathogen species and with the Spirochaetazuelaera. Tannerella forsythia shows its evolutionary relationship only with four oral pathogens (Macellibacteroidesfermentans, Porphyromadaceae bacterium, Parabacteroidesmeredae and Bacillus fosythus). Prevotellaintermedia also showed its evolutionary relationship only with Prevotella Spcs while Fusobacterium revealed close evolutionary relationship only with Porpiromonasgingivalis.
Keywords	Periodontal diseases, Diabetes, Microbial pathogens, PCR
Citation	Chiranjeevi et al. Bioinformation 10(4): 241-245 (2014)
Edited by	P Kangueane
ISSN	0973-2063
Publisher	Biomedical Informatics
License	This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.