Title

Analysis of predicted B and T-cell epitopes in Der p 23, allergen from Dermatophagoides pteronyssinus

Authors

Songwe Fanuel^{1, 2}, Saeideh Tabesh³, Esmaeil Sadroddiny¹, Gholam Ali Kardar^{1, 2*}

Affiliation

¹Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences-International Campus (IC-TUMS) Tehran University of Medical Sciences, Tehran, Iran;

²Immunology, Asthma & Allergy Research Institute (IAARI), Tehran, Iran;
³Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.

Email

gakardar@tums.ac.ir

Article Type

Hypothesis

Date

Received August 10, 2017; Revised September 4, 2017; Accepted September 6, 2017; Published September 30, 2017

House dust mite (HDM) allergy is the leading cause of IgE-mediated hypersensitivity. Therefore identifying potential epitopes in the Dermatophagoide pteronyssinus 23 (Der p 23), a major house dust mite allergen will aid in the development of therapeutic vaccines and diagnostic kits for HDM allergy. Experimental methods of epitope discovery have been widely exploited for the mapping of potential allergens. This study sought to use immunoinformatic methods to analyze the structure of Der p 23 for potential immunoreactive B and T-cell epitopes that could be useful for AIT and allergy diagnosis. We retrieved a Der p 23 allergen sequence from Genbank database and then analyzed it using a combination of web-based sequence analysis tools including the Immune Epitope Database (IEDB), Protparam, BCPREDS, ABCpred, BepiPred, Bcepred among others to predict the physiochemical properties and epitope spectra of the Der p 23 allergen. We then built 3D models of the predicted B-cell epitopes, T cell epitopes and Der p 23 for sequence structure homology analysis. Our results identified peptides ‘TRWNEDE’, ‘TVHPTTTEQPDDK’, and ‘NDDDPTT’ as immunogenic linear B-cell epitopes while ‘CPSRFGYFADPKDPH’ and ‘CPGNTRWNEDEETCT’ were found to be the most suitable T-cell epitopes that interacted well with a large number of MHC II alleles. Both epitopes had high population coverage as well as showing a 100% conservancy. These five Der p 23 epitopes are useful for AIT vaccines and HDM allergy diagnosis development.

Keywords

Epitopes, Der p 23, Allergen Immunotherapy, House Dust Mite, immunoinformatics

Citation

Fanuel et al. Bioinformation 13(9): 307-312 (2017)

Edited by

P Kangueane

ISSN

0973-2063

Publisher

Biomedical Informatics

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.