Title

Towards the construction of an interactome for Human WD40 protein family

Authors

Hulikal Shivashankara Santosh kumar1, Vadlapudi Kumar2*, Sharath Pattar3, Sandeep Telkar4

Affiliation

1Department of Biotechnology and Bioinformatics, Kuvempu University, Shankaraghatta-577451, Karnataka, India; 2Department of Biochemistry, Davanagere University, Shivagangothri, Davanagere-577002, Karnataka, India; 3National Bureau of Agriculturally Important Insects, Hebbal, Bengaluru, Karnataka, India; 4Department of Biotechnology and Bioinformatics, Kuvempu University, Shankaraghatta-577451, Karnataka, India;

Email

Vadlapudi Kumar - Email: vadlapudikumar@gmail.com; *Corresponding author

Article Type

Hypothesis

Date

Received February 17, 2016; Revised February 25, 2016; Accepted February 25, 2016; Published April 10, 2016

WD40 proteins are involved in a variety of protein-protein interactions as part of a multi-protein assembly modulating diverse and critical cellular process. It is known that several proteins of this family have been implicated in different disorders such as developmental abnormalities and cancer. However, molecular functions of many proteins in this family are yet unknown and it is of clinical interest. Therefore, it is of interest to define, construct, understand, analyze, evaluate, redefine and refine an interactome for WD40 protein family. We used data from literature mining using Cytoscape followed by linear regression analysis between Betweenness centrality and stress scores to define a model to filter the nodes in a representative WD40 interactome construction. We identified 10 ranked nodes in this analysis and subsequent microarray data selected three of them in insulin resistance that is further demonstrated in HepG2 cell culture models. We also observed the expression of GRWD1, RBBP5 and WDR5 genes during perturbation. Thus, we report hub nodes of WD40 interactome in insulin resistance. It should be noted that the pipeline using protein interaction network help find new proteins of clinical importance.

WDR5, Interactome, Betweenness Centrality, Stress, Insluin Resistance

Citation

Kumar et al. Bioinformation 12(2): 54-61 (2016)
 

Edited by

P Kangueane

ISSN

0973-2063

Publisher

Biomedical Informatics

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.