The Potential effect of G915C polymorphism in regulating TGF-β1 transport into Endoplasmic Reticulum for cytokine production

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Title	The Potential effect of G915C polymorphism in regulating TGF-β1 transport into Endoplasmic Reticulum for cytokine production
Authors	Hani Susianti¹*, Atma Gunawan², Jayarani Fatimah Putri³, Basuki B Purnomo⁴ Kusworini Handono¹ & Handono Kalim²
Affiliation	¹Departement of Clinical Pathology, Faculty of Medicine, Brawijaya University, Malang, Indonesia; ²Departement of Internal Medicine, Faculty of Medicine, Brawijaya University, Malang, Indonesia; ³Departement of Biology, Faculty of Sciences, Brawijaya University, Malang, Indonesia; ⁴Department of Urology, Faculty of Medicine, Brawijaya University, Malang, Indonesia
Email	hanisusianti.fk@ub.ac.id; *Corresponding author
Article Type	Hypothesis
Date	Received July 17, 2014; Accepted July 26, 2014; Published August 30, 2014
Abstract	The TGF-β1 cytokine concentration is known to be higher in nephritis with implied Lupus Nephritis severity. The production of TGF-β1 cytokine is associated with G915C polymorphism. Therefore, it is of interest to study G915C polymorphism. The G915C polymorphism changes codon 25 which encodes arginine into proline in the signal peptide of TGF-β1. The amino acid substitution affects signal peptide properties that may inhibit the transport of TGF-β1 into the endoplasmic reticulum and eventually decline the cytokine production. Hence, the effect of G915C polymorphism on the properties of the signal peptide, the ability of TGF-β1 transport into the endoplasmic reticulum and the concentrations of urinary TGF-β1 in Lupus Nephritis patients was studied. The arginine substitution into proline decreased the polarity of the signal peptide for TGF-β1. The increased hydrophobicity with increased binding energy of the signal peptide for TGF-β1 to Signal Recognition Particle (SRP) and translocon is shown. This implies decreased protein complex stability in potentially blocking the transport of TGF-β1 into the endoplasmic reticulum. This transport retention possibly hampers the synthesis and maturation of TGF-β1 leading to decreased cytokine production.
Citation	Susianti et al. Bioinformation 10(8): 487-490 (2014)
Edited by	P Kangueane
ISSN	0973-2063
Publisher	Biomedical Informatics
License	This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.