BACK TO CONTENTS   |    PDF   |    PREVIOUS   |   


Identification of Microbial Pathogens in Periodontal disease and Diabetic patients of South Indian Population



Tikka Chiranjeevi1, Osuru Hari Prasad1, Uppu Venkateswara Prasad2, Avula Kishor Kumar3, Veeraraghavulu Praveen Chakravarthi1, Paramala Balaji Rao1, Potuguchi Venkata Gurunadha Krishna Sarma2, Nagi reddy Raveendra Reddy3, Matcha Bhaskar1*



1Division of Animal Biotechnology, Department of Zoology, S. V. University, Tirupati-517502, Andhra Pradesh, India; 2Department of Biotechnology, SVIMS, Tirupati-517502, Andhra Pradesh, India; 3Department of Periodontology, Krishna Teja Dental College, Tirupati, Andhra Pradesh, India

Email; *Corresponding authors


Article Type

Research note



Received March 07, 2014; Accepted March 14, 2014; Published April 29, 2014


Periodontitis have been referred to as the sixth complication of diabetes found in high prevalence among diabetic patients than among healthy controls. The aim of the present study was to examine the periodontal disease status among collected dental plaque samples. Chromosomal DNA was isolated and amplified by universal primers. The DNA was sequenced for bacterial confirmation and phylogenetic analysis performed for the evolutionary relationship with other known pathogens. No amplification products were observed in groups labeled non periodontal and non Diabetes (NP&ND) and non Periodontal and Diabetes (NP&D). But in the case of Periodontal and non Diabetes (P&ND) groups 22 amplified products were observed. In case of Periodontal and Diabetes (P&D), 32 amplified products were positive for microbes. Among the four microbial groups, Treponemadenticola, and Tannerella forsythia were found to be prevalent in P&D. The phylogenetic analysis of 16s rRNA of Treponemadenticola showed the relationship with other Treponema oral pathogen species and with the Spirochaetazuelaera. Tannerella forsythia shows its evolutionary relationship only with four oral pathogens (Macellibacteroidesfermentans, Porphyromadaceae bacterium, Parabacteroidesmeredae and Bacillus fosythus). Prevotellaintermedia also showed its evolutionary relationship only with Prevotella Spcs while Fusobacterium revealed close evolutionary relationship only with Porpiromonasgingivalis.



Periodontal diseases, Diabetes, Microbial pathogens, PCR



Chiranjeevi et al. Bioinformation 10(4): 241-245 (2014)


Edited by

P Kangueane






Biomedical Informatics



This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.