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Title

PCR-based molecular characterization, phylogenetic analysis and secondary structure of the 28S rDNA of Thaparocleidus wallagonius (Monogenea: Dactylogyridae) the most primitive species of this genus from India

 

Authors

Chandni Verma, Anshu Chaudhary* & Hridaya Shanker Singh

 

Affiliation

Molecular Taxonomy Laboratory, Department of Zoology, University Road, C. C. S. University, Meerut (UP), 250004, India.

 

Email

anshu8282@rediffmail.com; *Corresponding author

 

Article Type

Hypothesis

 

Date

Received August 19, 2012; Accepted August 20, 2012; Published September 11, 2012

 

Abstract

Species of the monogenean genus Thaparocleidus are specific to freshwater siluriform fish. The infection caused by these gill parasites are a major health problem to fish. But, to focus the control strategies of these parasites, first it is important to establish an accurate discrimination by molecular methods. In the present study, phylogenetic and structural analysis of 28S region of ribosomal DNA of T. wallagonius species collected from fish Wallago attu from Meerut (U.P.), India, was carried out. In the first step, we amplified, sequenced 28S region of ribosomal DNA of T. wallagonius to establish the phylogenetic relationship with other species of this genus. T. wallagonius found on gill filaments of fish W. attu, is the most primitive parasite of this genus from India, was unequivocally discriminate from other species of the same genus in this study. A secondary-structure model of the large subunit rDNA was also predicted using a combined comparative and thermodynamic approach. Molecular morphometric and phylogenetic relationship of T. wallagonius are discussed in detailed that based on molecular analysis using bioinformatic tools.

 

Keywords

Monogenea, 28S, secondary structure, India, ribosomal DNA

 

Citation

Verma et al. Bioinformation 8(17): 816-819 (2012)
 

Edited by

P Kangueane

 

ISSN

0973-2063

 

Publisher

Biomedical Informatics

 

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.