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Title

Identification of Escherichia coli through analysis of 16S rRNA and 16S-23S rRNA internal transcribed spacer region sequences

 

Authors

Mohammed Siraj Ud Din Magray1, Anup Kumar2, Anil Kumar Rawat1, Shipra Srivastava1*

 

Affiliation

1Biotechnology and Bioinformatics Division, BIOBRAINZ, 566/29 J, Jai Prakash Nagar, Alambagh, Lucknow 226005, Uttar Pradesh, India; 2Department of Applied Sciences, Sherwood college of Engineering Research & Technology, Lucknow, Uttar Pradesh, India

 

Email

shipra.srivastava09@gmail.com; *Corresponding author

 

Phone

91- 0522 4042284

 

Article Type

Hypothesis

 

Date

Received May 23, 2011; Accepted May 26, 2011; Published August 02, 2011

 

Abstract

A bacterial strain, designated BzDS03 was isolated from water sample, collected from Dal Lake Srinagar. The strain was characterized by using 16S ribosomal RNA gene and 16S-23S rRNA internal transcribed spacer region sequences. Phylogenetic analysis showed that 16S rRNA sequence of the isolate formed a monophyletic clade with genera Escherichia. The closest phylogenetic relative was Escherichia coli with 99% 16S rRNA gene sequence similarity. The result of Ribosomal database projectís classifier tool revealed that the strain BzDS03 belongs to genera Escherichia.16S rRNA sequence of isolate was deposited in GenBank with accession number FJ961336. Further analysis of 16S-23S rRNA sequence of isolate confirms that the identified strain BzDS03 be assigned as the type strain of Escherichia coli with 98% 16S-23S rRNA sequence similarity. The GenBank accession number allotted for 16S-23S rRNA intergenic spacer sequence of isolate is FJ961337.

 

Keywords

16S ribosomal RNA gene; 16S-23S intergenic spacer region; Escherichia coli; Phylogenetic analysis; DNA isolation; DNA sequencing

 

Citation

Magray et al. Bioinformation 6(10): 370-371 (2011)
 

Edited by

P Kangueane

 

ISSN

0973-2063

 

Publisher

Biomedical Informatics

 

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.