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Title

Isolation, identification and computational studies on Pseudomonas aeruginosa sp. strain MPC1 in tannery effluent

 

Authors

Renganathan Senthil*, Kanagamani Jini Angel, Ravi Malathi, Dhanapal Venkatesan

 

Affiliation

Department of Bioinformatics, Marudupandiyar Institutions, Thanjavur-613403, TamilNadu, India

 

Email

renganathansenthil@gmail.com; *Corresponding author

 

Phone

04362-266791, 265014, 650902

 

Fax

265191

 

Article Type

Hypothesis

 

Date

Received April 20, 2011; Accepted May 07, 2011; Published May 26, 2011

 

Abstract

A study about isolation, identification and analysis of bacteria in waste water. Here the tannery effluent used as a sample for the entire analysis. A bacterial strain, designated MPC1 was isolated from a waste water sample collected from tannery effluent, Trichy, India and identified using a molecular approach. On the basis of the bacterial 16s rRNA gene sequence phylogeny and comparison of this gene sequence with sequence in RNA sequence database, it is considered that isolate is closely related to members of the Pseudomonas aeruginosa Sp. Phylogenetic and molecular evolutionary analyses were conducted using MEGA. Identification of regulatory elements and Transcription Factor with their binding sites in 16S rRNA gene of Pseudomonas aeruginosa mpc1 was performed using BPROM tool. The sequence of 16s rRNA (Pseudomonas aeruginosa sp MPC 1) is submitted to Genbank in NCBI database (Ac.No-JF708077).

 

Keywords

Pseudomonas aeruginosa sp.Strain MPC1, isolation, identification, in silico analysis, Heavy metal resistant bacteria.

 

Citation

Senthil et al. Bioinformation 6(5): 187-190 (2011)

Edited by

P Kangueane

 

ISSN

0973-2063

 

Publisher

Biomedical Informatics

 

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.