Title

AP-APSE dpol intein: A novel family A DNA polymerase intein domain.

Authors

Senguttuvan Rajarajan, Kalibulla Syed Ibrahim, Shunmugiah Karutha Pandian*

Affiliation

Bioinformatics Infrastructure Facility, Department of Biotechnology, Alagappa University, Karaikudi-630003, India

Email

sk_pandian@rediffmail.com; *Corresponding author

Article Type

Hypothesis

Date

Received September 28, 2010; Accepted February 03, 2011; Published May 07, 2011.

Inteins are "protein introns" that remove themselves from their host proteins through an autocatalytic protein-splicing. After their discovery, inteins have been quickly identified in organisms from all three kingdoms of life - eucarya, bacteria and archaea, but their distribution is sporadic. Here we report the identification and bioinformatics characterization of intein in DNA polymerase A gene of bacteriophage APSE (Acyrthosiphon pisum Secondary Endosymbiont bacteriophage) infecting the Aphid secondary endosymbionts of eukaryotic insects such as Acyrthosiphon pisum, Uroleucon rudbeckiae. The insertion site of intein within APSE family A DNA polymerase extein was identified to be dpola. Hence we propose this as a unique intein of family A DNA polymerase (dpola insertion site) and only reported intein in podoviridae family.

Citation

Rajarajan et al. Bioinformation 6(4): 149-152 (2011)

Edited by

N Gautham

ISSN

0973-2063

Publisher

Biomedical Informatics

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.