Title

Dataset of potential targets for Mycobacterium tuberculosis H37Rv through comparative genome analysis
 

Authors

Siddiqui M. Asif^1*, Amir Asad¹, Ahmad Faizan², Malik S. Anjali¹, Arya Arvind¹, Kapoor Neelesh¹, Kumar Hirdesh¹, Kumar Sanjay²

Affiliation

¹Department of Biotechnology, Meerut Institute of Engineering and Technology, Meerut; ²Department of Biotechnology, Shobhit University, Meerut

Email

asifsiddiqui82@gmail.com

Article Type

Hypothesis

Date

Received November 19, 2009; Accepted December 14, 2009; Published December 31, 2009

Abstract

Mycobacterium tuberculosis is the causative agent of the disease, tuberculosis and H37Rv is the most studied clinical strain. We use comparative genome analysis of Mycobacterium tuberculosis H37Rv and human for the identification of potential targets dataset. We used DEG (Database of Essential Genes) to identify essential genes in the H37Rv strain. The analysis shows that 628 of the 3989 genes in Mycobacterium tuberculosis H37Rv were found to be essential of which 324 genes lack similarity to the human genome. Subsequently hypothetical proteins were removed through manual curation. This further resulted in a dataset of 135 proteins with essential function and no homology to human.

Keywords

Mycobacterium tuberculosis H37Rv, DEG, BLASTX, targets

Citation

Asif et al., Bioinformation 4(6): 245-248 (2009)

Edited by

P. Kangueane

ISSN

0973-2063

Publisher

Biomedical Informatics

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.