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Title

 

 

 

 

 

A molecular model for diacylglycerol acyltransferase from Mortierella ramanniana var. angulispora

 

Authors

 

Sanjay Mishra1,*, Surya Prakash Dwivedi1, Neeraja Dwivedi1, Ajay Kumar1, Anil Rawat2 and Yasushi Kamisaka3

 

Affiliation

1Department of Biotechnology, College of Engineering and Technology, IFTM Campus, Lodhipur-Rajput, Delhi Road, Moradabad 244 001, U.P., India; 2Bioinformatics Laboratory, Biobranz Institute, Lucknow 226 001, U.P., India; 3Lipid Engineering Research Group Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki 305-8566 Japan

 

Email

 

sanjaymishra66@gmail.com

Article Type

 

Hypothesis

Date

 

received December 10, 2008; revised February 19 2009; accepted April 14, 2009; published June 28, 2009

Abstract

Acyl Co-A diacylglycerol acyltransferase (DGAT, EC 2.3.120) is recognized as a key player of cellular diacylglycerol metabolism. It catalyzes the terminal, yet the committed step in triacylglycerol synthesis using diacylglycerol and fatty acyl CoA as substrates. The protein sequence of diacylglycerol acyltransferse (DGAT) Type 2B in Moretierella ramanniana var. angulispora (Protein_ID = AAK84180.1) was retrieved from GenBank. However, a structure is not yet available for this sequence. The 3D structure of DGAT Type 2B was modeled using a template structure (PDB ID: 1K30) obtained from Protein databank (PDB) identified by searching with position specific iterative BLAST (PSI-BLAST). The template (PDB ID: 1K30) describes the structure of DGAT from Cucurbita moschata. Modeling was performed using Modeller 9v2 and protein model is hence generated. The DGAT type 2B protein model was subsequently docked with six inhibitors (sphingosine; trifluoroperazine; phosphatidic acid; lysophospatidylserine; KCl; 1, 2-diolein) using AutoDock (a molecular docking program). The binding of inhibitors to the protein model of DGAT type 2B is discussed.

 

Keywords

AutoDock; DGAT; diacylglycerol (DG); Mortierella ramanniana; Modeller; PDB; phosphatidic acid (PA); PSIBLAST;RMSD; triacylglycerol (TG)

Citation

 

Mishra et al, Bioinformation 3(9): 394-398 (2009)

Edited by

P. Kangueane

ISSN

0973-2063

 

Publisher

Biomedical Informatics

License

 

 

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.