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Title |
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Alternatively spliced isoforms encoded by cadherin genes from C. elegans genome
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Authors |
Luv Kashyap and Mohammad Tabish* |
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Affiliation |
Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, India |
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+91 571 2700741; * Corresponding author |
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Article Type |
Hypothesis
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Date |
received July 27, 2007; revised September 05, 2007; accepted September 11, 2007; published online September 24, 2007
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Abstract |
Cadherins are calcium-dependent, homophilic, cell-cell adhesion receptors that regulate morphogenesis, pattern formation and cell migration. The C. elegans Genome Sequencing Consortium has reported 12 genes from C. elegans genome encoding members of the cadherin superfamily. Alternative splicing of eukaryotic pre-mRNAs is a mechanism for generating potentially many transcript isoforms from a single gene. Here, using a combination of various gene/exon finding programmes and several other bioinformatics tools followed by experimental validation using RT-PCR, we have studied alternative splicing pattern in the cadherin encoding genes from C. elegans genome. We have predicted that 7 of the 12 genes encoding the cadherin superfamily undergo extensive alternative splicing and encode for 12 new unreported alternatively spliced transcripts. Most of the alternatively spliced exons were found to be present at the 5'end of genes. These new previously un-detected spliced variants in C. elegans cadherin superfamily of genes could play vital roles in explaining the way cadherins act to control the processes like cell adhesion and morphogenesis.
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Keywords
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cadherins; alternative splicing; computational analysis; exons; isoforms; C. elegans
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Citation |
Kashyap et al., Bioinformation 2(2): 50-56 (2007) |
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Edited by |
M. K. Sakharkar |
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ISSN |
0973-2063
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Publisher |
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License |
This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.
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