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Title |
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Computational and molecular characterization of multiple isoforms of lfe-2 gene in nematode C. elegans
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Authors |
Luv Kashyap1, Mohammad Tabish1*, Subramaniam Ganesh2, and Deepti Dubey2 |
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Affiliation |
1Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, India; 2Department of Biological Sciences and Bioengineering, Indian Institute of Technology, Kanpur, India
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tabish.biochem@gmail.com; * Corresponding author
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Phone |
+91-571-270-0741
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Article Type |
Hypothesis
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Date |
received May 01, 2007; accepted May
02, 2007; published online May 30, 2007 |
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Abstract |
C. elegans C46H11.4 gene encodes a Let-23 fertility effector/regulator protein of the EGF-receptor class of the tyrosine kinase family. Alternative splicing is a major mechanism of generating protein diversity in higher eukaryotes. C. elegans genome sequencing consortium has reported three alternatively spliced transcripts of C46H11.4 gene which encodes for three hypothetical proteins namely, C46H11.4a, C46H11.4b and C46H11.4c. Using a combination of various bioinformatics tools like gene/exon finding programmes, blast searches, alignment tools etc followed by experimental validation, we report the presence of three more alternatively spliced transcripts which encode for novel hypothetical proteins C46H11.4d, C46H11.4e and C46H11.4f. These isoforms arise as a result of alternative splicing in the pre-mRNA encoded by gene C46H11.4. These novel un-reported spliced variants not only point towards the extent of alternative splicing in C. elegans genes but also hint towards the complex nature of alternative splicing.
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Keywords
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lfe-2; Let-23 fertility effector/regulator protein; exon prediction; alternative splicing; C. elegans
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Citation |
Kashyap et al., Bioinformation 2(1): 17-21 (2007)
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Edited by |
P. Kangueane
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ISSN |
0973-2063
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Publisher |
Biomedical Informatics |
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License |
This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.
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