Title
Cytotoxic, antioxidant, antibacterial activity of phytochemicals from Phragmanthera austroarabica
Authors
Moodi S. A. Alsubeie1, Nasir A. Ibrahim1, Ahmed A. Alghamdi 2, Nosiba S. Basher1, BS Al-ammari1, Awadallah B. Dafaallah 2, 3 & Vajid Nettoor Veettil4, 5,*
Affiliation
1Department of Biology, Faculty of Science, Imam Mohammed Ibn Saud Islamic University, Riyadh, Saudi Arabia; 2National Center for Vegetation Cover Development and Combating Desertification (NCVC), Ministry of Environment, Water and Agriculture, Riyadh, Kingdom of Saudi Arabia; 3Department of Crop Protection, Faculty of Agricultural Sciences, University of Gezira, Wad Medani, Sudan; 4Iqraa Centre for Research and Development, IQRAA International Hospital and Research Centre, Kozhikode, Kerala, India; 5MHES College of Science and Technology, Kozhikode, Kerala, India; *Corresponding author
Moodi S. A. Alsubeie - E-mail: msalsubeie@imamu.edu.sa
Nasir A. Ibrahim - E-mail: naabdalneim@imamu.edu.sa
Ahmed A. Alghamdi - E-mail: ahaalghamdi@ncvc.gov.sa
Nosiba S. Basher - E-mail: nsbasher@ imamu.edu.sa; researchdirector@iqraahospital.in
BS Al-ammari - E-mail: bsalammari@imamu.edu.sa
Awadallah B. Dafaallah - E-mail: abelal@ncvc.gov.sa
Vajid Nettoor Veettil - E-mail: vajidnv@gmail.com; director@mhescollege.ac.in; Phone No: +91 9605555029
Article Type
Research Article
Date
Received May 1, 2024; Revised May 31, 2024; Accepted May 31, 2024, Published May 31, 2024
Abstract
The cytotoxic, antioxidant, anticancer, and antibacterial properties of ethanolic extracts from Phragmanthera austroarabica is of interest. Plants of P. austroarabica were gathered from the southern Saudi Arabian region of Albaha. P. austroarabica extract was assessed using DPPH (2, 2-diphenyl-1-picrylhydrazyl). The German Collection of Microorganisms and Cell Cultures (DSMZ) cancer cell lines used in this investigation. The cytotoxic activity of P. austroarabica extract was explored against MCF-7 breast and A549 lung cancer cell lines, along with doxorubicin as a positive control. In both treated cells, P. austroarabica showed a remarkable activity via suppressing the cell’s survival. In terms of IC50 (concentration equivalent to a survival rate of 50%), MCF-7 breast cancer cells were more sensitive to P. austroarabica extract.) DPPH colorimetric assay was employed to assess the antioxidant properties of P. austroarabica extract, the antioxidant activity was increased along with increment of extract concentrations. The leaves aqueous extract of P. austroarabica inhibited the growth of S. aureus by 6.3±0.12 mm and 24±0.43 mm and 15±0.56 mm respectively for seed, leaf and stem at concentrations 50 μl. However, the same concentrations inhibited the growth of E. coli by 25±0.75, 0.00 mm and 24±0.18 mm, following the same order. Different superscript letters indicate means that are significantly different at level (p˂0.05). Minimal inhibitory concentrations (MIC) of P. austroarabica ethanolic extracts against the tested microorganisms were 1.5, 1.6 and 1.5, respectively for seed, leaf and stem against Staph. Aureus and were 1.2, 0.00 and 1.2, respectively for seed, leaf and stem against E. coli.
Keywords
Cytotoxic effect; antioxidant; antibacterial activity; phytochemicals profile; P. austroarabica
Citation
Alsubeie et al. Bioinformation 20(5): 487-494 (2024)
Edited by
P Kangueane
ISSN
0973-2063
Publisher
License
This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.
