Title

Effect of novel IL-8 gene mutation on its protein structure and stability among ovarian cancer patients in Saudi Arabia

Authors

Absarul Haque^1,2*, Ghazanfar Ali Baig³, Abdulelah Saleh Alshawli^1,2, Khalid Hussain Wali Sait⁴, Hani S.H Mohammed Ali³, Hesham Khalid Hussain Sait⁴, Fehmida Bibi^2,5, Peter Natesan Pushparaj^2,6, Mahmood Rasool^2,6 & Muhammad Imran Naseer^2,6

Affiliation

¹King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia; ²Department of Medical Laboratory Sciences, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, Saudi Arabia; ³Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi Arabia; ⁴Department of Gynecology Oncology Unit, Obstetrics and Gynecology, Faculty of Medicine, King Abdulaziz University Hospital, Jeddah, Saudi Arabia; ⁵Special Infectious Agents Unit, King Fahd Medical Research Centre, King Abdulaziz University, Jeddah, Saudi Arabia; ⁶Institute of Genomic Medicine Sciences, King Abdulaziz University, Jeddah, Saudi Arabia; *Corresponding author

Email

Absarul Haque - E - mail: mhaque@kau.edu.sa; Phone: +966126401000

Ghazanfar Ali Baig - E - mail: gbaig@stu.kau.edu.sa

Abdulelah Saleh Alshawli - E - mail: aalshawli@kau.edu.sa

Khalid Hussain Wali Sait - E - mail: khsait@kau.edu.sa

Hani S.H Mohammed Ali - E - mail: hmohammedali@kau.edu.sa

Hesham Khalid Hussain Sait - E - mail: heshamsait@yahoo.com

Fehmida Bibi - E - mail: fnaseer@kau.edu.sa

Peter Natesan Pushparaj - E - mail: pnatesan@kau.edu.sa

Mahmood Rasool - E - mail: mrahmed1@kau.edu.sa

Muhammad Imran Naseer - E - mail: minaseer@kau.edu.sa

Article Type

Research Article

Date

Received November 1, 2024; Revised November 30, 2024; Accepted November 30, 2024, Published November 30, 2024

Abstract

Despite advancements in therapeutics, early diagnosis of ovarian cancer (OC) remains a challenge due to its asymptomatic progression and it is often diagnosed at an advanced stage. Interleukin-8 (IL-8), a pro-inflammatory chemokine with angiogenic activity, plays a pivotal role in inflammation and carcinogenesis. Therefore, it is of interest to investigate the association between IL-8 polymorphisms and OC risk. A novel non-synonymous SNP (nsSNP, c.193G>A; p.Glu65Leu) was identified in exon 2 of the IL-8 gene in 5 patients, 3 with stage III and 2 with stage II tumor. Structural analysis suggested that the p.Glu65Leu mutation alters the IL 8 protein conformation showing functional impairment. Thus, data shows that IL-8 c.193 G>A (Glu65Leu) polymorphism may contribute to tumor susceptibility. However, further studies are warranted to understand the role of IL-8 polymorphism in OC.

Keywords

Ovarian cancer, Interleukin-8, Single Nucleotide Polymorphism, automated DNA sequencing, inflammation

Citation

Haque et al. Bioinformation 20(11): 1671-1677 (2024)

Edited by

P Kangueane

ISSN

0973-2063

Publisher

Biomedical Informatics

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.