Title
Identification and quantification of lipid accumulation in adipose tissue using oil red O and Sudan stains
Authors
Swati Shrivastava1, Aishwarya Srivastava2, Ruchi Jain3, Amit Kumar Srivastava4,*, Sourabh Shrivastava5 & Rashmi Sathe6
Affiliation
1Department of Biochemistry, Government Medical College, Datia, Madhya Pradesh, India; 2Department of Biochemistry, All India Institute of Medical Sciences, Gorakhpur, Uttar Pradesh, India; 3Department of Anatomy, MLB Medical College, Jhansi, Uttar Pradesh, India; 4Department of Anatomy, Government Medical College Datia, Madhya Pradesh, India; 5Department of Anaesthesiology, GAJRA Raja Medical College, Gwalior, Madhya Pradesh, India; 6Department of Oral Medicine and Radiology, People's College of Dental Sciences and Research Centre, Bhopal, Madhya Pradesh, India; *Corresponding Author
Swati Shrivastava - E - mail: swatishrivastava574@gmail.com
Aishwarya Srivastava - E - mail: aishwaryasrivastava720@gmail.com
Ruchi Jain - E - mail: drruchijain2012@gmail.com
Amit Kumar Srivastava - E - mail: amitsrivastava48@gmail.com
Sourabh Shrivastava - E - mail: sourabh_jitu@ymail.com
Rashmi Sathe - E - mail: rashmissathe@gmail.com
Article Type
Research Article
Date
Received October 1, 2024; Revised November 5, 2024; Accepted November 5, 2024, Published November 5, 2024
Abstract
This study investigates the application of Oil Red O and Sudan stains in identifying and quantifying lipid accumulation in adipose tissue, specifically focusing on its relevance to obesity-related oral diseases. Adipose tissue samples were collected from 50 obese patients (BMI > 30) and 50 normal-weight controls. Samples were stained with Oil Red O and Sudan III, IV and Black. Lipid accumulation was quantified using digital image analysis. Oral health examinations assessed the prevalence of periodontal disease, dental caries, and oral candidiasis. Oil Red O staining show 2.8-fold higher lipid contents obese subjects than controls (p<0.001). Sudan stains demonstrated similar trends, with Sudan Black being the most sensitive (3.2-fold increase, p<0.001). Positive correlations were found between lipid accumulation and the severity of periodontal disease (r=0.72, p<0.001), dental caries (r=0.58, p<0.01), and oral candidiasis (r=0.63, p<0.01) in obese subjects. Oil Red O and Sudan stains effectively identify and quantify lipid accumulation in adipose tissue. The study's findings underscore a robust link between increased lipid content and the prevalence of obesity-related oral diseases, highlighting the potential of these staining techniques in oral health research and clinical practice.
Keywords
Oil Red O; Sudan stains; adipose tissue, obesity & oral diseases
Citation
Shrivastava et al. Bioinformation 20(11): 1663-1666 (2024)
Edited by
Vini Mehta
ISSN
0973-2063
Publisher
License
This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.
