Title
In vitro anti-cancer activity of siddha metallo mineral formulation from Thanga uram with Hela cell lines
Authors
Sathya Rathish Mohana Jeevanath1,*, Manjari Venkatraman1, Kalaivanan Karuppan2, Abarna Balasubramani1, Natarajan Mohan1 & Siva Annamalai3
Affiliation
1Department of Nanju Maruthuvam, National Institute of Siddha, Dr.M.G.R Medical University, Chennai - 47, Tamil Nadu, India; 2Department of Siddha Medicine, Government Primary Health Centre, Perungattur, Tiruvannamalai - 604402, Tamil Nadu, India; 3Department of Clinical Research, Siddha Regional Research Institute (Central Council for Research in Siddha, Ministry of Ayush, Govt. of India) Kuyavarpalayam, Puducherry, India; *Corresponding author
Sathya Rathish Mohana Jeevanath - E - mail: jeevanath.rathish@gmail.com
Manjari Venkatraman - E - mail: drmanjarimd@gmail.com
Kalaivanan Karuppan - E - mail: drkalaimds@gmail.com
Abarna Balasubramani - E - mail: abu.abarna96@gmail.com
Natarajan Mohan - E - mail: natarajandbz@gmail.com
Siva Annamalai - E - mail: raimrtcsrri@gmail.com
Article Type
Research Article
Date
Received November 1, 2024; Revised November 30, 2024; Accepted November 30, 2024, Published November 30, 2024
Abstract
The second most common malignant tumour in women worldwide, cervical cancer seriously jeopardizes their health. It has been established that a high-risk Human papillomavirus (HPV) infection is the cause of cervical cancer. Cervical cancer is associated with several risk factors, including reproductive, behavioural and sexual characteristics. Many Siddha practitioners often prescribe Thanga uram (TU), a Siddha metallo-mineral formulation, for genito-urinary diseases, infertility, varicose veins and leucorrhoea. This study aims to evaluate the anti-cancer activity of "Thanga uram" against HeLa cell lines. Hela cell lines were used to assess the antiproliferative activity TU using the MTT assay and apoptosis by Annexin V flow cytometry. Thanga uram produced dose dependent cytotoxic effects against HeLa cell lines with an LC50 value of 145.891µg/mL. Further investigation, using Annexin V flow cytometry, equivalently revealed the late apoptotic effect, with a percentage value of 28.15. Thus, the current study evaluates the effectiveness of Thanga Uram against cervical cancer.
Keywords
Anti-cancer, annexin V flowcytometry, HeLa cell line, MTT assay, siddha, thanga uram
Citation
Jeevanath et al. Bioinformation 20(11): 1603-1607 (2024)
Edited by
P Kangueane
ISSN
0973-2063
Publisher
License
This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.
