Title
Clinical characterization, molecular and genomic sequencing analysis of SARS-Cov-2 during second wave at Raigarh, Chhattisgarh, India
Authors
Anubha Patel1, Anuniti Mathias2, Ashish Baghel3,*, Ankita Kumari4 & Swati Kujur5
Affiliation
1Department of Microbiology, Government Medical College, Mahasamund, Chhattisgarh, India; 2Department of Microbiology, BRLSABVM Medical College, Rajnandgaon, Chhattisgarh, India; 3Department of Community Medicine, Government Medical College, Mahasamund, Chhattisgarh, India; 4Department of Microbiology, LSLAMG Medical College, Raigarh, Chhattisgarh, India; 5Scientific Officer (Biology), State Forensic Science Laboratory, Raipur, Chhattisgarh, India; *Corresponding author
Anubha Patel - E - mail: dr.aana99@gmail.com
Anuniti Mathias - E - mail: anunitimathias@gmail.com
Ashish Baghel - E - mail: dr.ashish9999@gmail.com
Ankita Kumari - E - mail: ankita16sah@gmail.com
Swati Kujur - E - mail: kujurswati@gmail.com
Article Type
Research Article
Date
Received September 1, 2024; Revised September 30, 2024; Accepted September 30, 2024, Published September 30, 2024
Abstract
The severe acute respiratory syndrome coronavirus-2 (SARS-Cov-2) has been changing continuously. This study was conducted to evaluate clinical characteristics, Molecular analysis & Genomic sequencing of SARS-Cov-2 during second wave in Raigarh district, Chhattisgarh, India. This study evaluated 13402 breakthrough cases of COVID -19. The laboratory obtained the nasopharyngeal/oropharyngeal swabs (NPS/OPS) of SARS-CoV-2 patients who tested positive by real-time RT-PCR, together with clinical and demographic information. Next generation sequencing (NGS) was used to sequence these clinical specimens in order to identify nucleotide changes in the SARS-CoV-2 genome from these strains. In the study population, variants of concern (VOCs) and other variations were looked for. Clinical severity was mild in 47.05% patients with mutational variants; while 52.94% patient's clinical severity was moderate. Delta (B.1.617.2) was the most common VOC detected. Among non VOC variants, AY.4 and AY.12 variants were most commonly detected. Envelope (E) gene and RNA-dependent RNA polymerase (RdRp) mutation were most commonly observed.
Keywords
SARS-Cov-2, second wave, COVID -19 Molecular analysis & Genomic sequencing.
Citation
Patel et al. Bioinformation 20(9): 1059-1064 (2024)
Edited by
P Kangueane
ISSN
0973-2063
Publisher
License
This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.
