Title
Early diagnosis of oral submucous fibrosis using salivary 8-OHDG and 8-Isoprostane
Authors
Bireswar Roy1, Shaili Ghosh2,*, SS Mohamed Abdulcader Riyaz3, Madhvika Patidar4, Urmi Mehta5, Priya Agarwal6 & Pushkar Gupta7
Affiliation
1Department of Oral Pathology and Microbiology, Sudha Rustagi College of Dental Sciences and Research, Faridabad, Haryana, India; 2Kalinga Institute of Dental Sciences, Bhubaneswar, Odisha, India; 3Department of Oral and Maxillofacial Diagnostic Sciences, College of Dentistry, Ar Rass Branch, Qassim University, Saudi Arabia; 4Department of Oral Pathology and Microbiology, Government College of Dentistry, Indore, MP, India; 5Dental Surgeon, Gujarat, India; 6Department of Oral Medicine and Radiology, Modern Dental College and Research Centre, Indore, MP, India; 7Department of Prosthodontics and Crown & Bridge, Hitkarini Dental College and Hospital, Jabalpur, MP, India; *Corresponding author
Bireswar Roy - E - mail: bireswarr12@gmail.com; Phone no: +91 9678796279
Shaili Ghosh - E - mail: shaili.g@hotmail.com ; Phone no: +91 9830122066
Mohamed Abdulcader Riyaz SS - E - mail: m.salahudeen@qu.edu.sa; Phone no: +966 507769863
Madhvika Patidar - E - mail: madhvikapatidar@gmail.com; Phone no: +91 9565477630
Urmi Mehta - E - mail: mehta.urmi9@gmail.com; Phone no: +91 9974568312
Priya Agarwal - E - mail: drpriya.agarwal10@gmail.com; Phone no: +91 9993022685
Pushkar Gupta- E - mail: drpushkar1@gmail.com; Phone no: +91 9406538208
Article Type
Research Article
Date
Received September 1, 2024; Revised September 30, 2024; Accepted September 30, 2024, Published September 30, 2024
Abstract
Oral submucous fibrosis (OSMF) is a condition that may be cancerous. The prognosis of OSMF is determined by a number of biomarkers, including 8-hydroxy 2′ de-oxy-guanosine and 8-isoprostane. It is possible to assess the levels of 8-OHdG and 8-isoprostane in blood and saliva. Therefore, it is of interest to estimate salivary 8-OHdG and 8-isoprostane levels in order to diagnose oral submucous fibrosis. A sample size of 40 was divided into two groups with 20 samples in each, i.e., Group I - Healthy group (gutka consumers without any lesion) and Group II -Test (gutka consumers with OSMF). Samples of serum and saliva were taken from each group. Then samples were centrifuged for 15–20 minutes at 1000 RPM and 2-8°C. The resulting supernatant was pipetted out into labelled Eppendorf tubes in a volume of 1.5 ml, and it was then kept at 80°C. 8-OHdG and 8-isoprostane concentrations in various samples were determined using the ELISA technique. Serum's 8-OHdG content was considerably lower than saliva sample (P-value <0.05). The test group exhibited increased concentrations of 8-OHdG and 8-isoprostane in both saliva and serum samples when compared to the control group. 8-OHdG and 8-isoprostane can be utilised to diagnoses of OSMF.
Keywords
DNA damage, gutka chewing oxidative stress, OSMF, saliva, 8-OHdG & 8-Isoprostane.
Citation
Roy et al. Bioinformation 20(9): 1042-1045 (2024)
Edited by
P Kangueane
ISSN
0973-2063
Publisher
License
This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.
