Title
Anti-arthritic efficacy of Bombax ceiba ethanolic extract in a murine model for rheumatoid arthritis using in vivo, in vitro and radiological analysis
Authors
Bhargavi Posinasetty1, Radhika Chikatipalli2*, Santenna Chenchula3, Kuttiappan Anitha4, G. Swapna2, G.N.A. Lakshmi2 & Audinarayana Nelavala2
Affiliation
1Department of Clinical Data Management, Prometrika LLC, Cambridge – MA 02140, United States; 2Department of Pharmacology, Sri Venkateswara College of Pharmacy, Chittoor - 517127, A.P., India; 3Department of clinical pharmacology, All India Institute of Medical Sciences, Bhopal, India, 462020; 4Amity Institute of Pharmacy, Amity University Madhya Pradesh (AUMP), Gwalior - 474005, Madhya Pradesh, India; *Corresponding author
Bhargavi Posinasetty – E-mail:Posinasettybhargavi@gmail.com
Radhika Chikatipalli - E-mail: drradhika@svcop.in
Santenna Chenchula – E-mail: csanten7@gmail.com
Kuttiappan Anitha – E-mail: kanitha@gwa.amity.edu
G. Swapna – E-mail: swapna@svcop.in
G.N.A. Lakshmi – E-mail: alakshmi@svcop.in
Audinarayana Nelavala – E-mail: audinarayana@svcop.in
Article Type
Research Article
Date
Received August 1, 2023; Revised August 31, 2023; Accepted August 31, 2023, Published August 31, 2023
Abstract
Rheumatoid arthritis (RA) is a multisystem autoimmune disease that causes discomfort, synovial membrane inflammation, peripheral joint inflammation, morning stiffness, articular tissue loss, and restricted joint movement. In the present study, we aim to explore the anti-arthritic efficacy of Bombax ceiba ethanolic extract in a Freund's Complete Adjuvant-induced arthritis, in murine model. The hot soxhlet method was used to extract dried aerial components of Bombax ceiba using an ethyl alcohol: water (70:30) ratio. Bombax ceiba ethanolic extract at two doses of 200 and 400 mg/kg was investigated in Wistar rats against Freund's full adjuvant-induced chronic immunological arthritis. Anti-arthritis efficacy was studied utilising morphological research (paw volume, paw diameter, and body weight). On the 28th day, the animals were sacrificed, and haematological parameters, pro-inflammatory cytokines (TNF-alpha, IL-6), cell culture, histological and radiological analysis were performed. BCEE inhibited paw oedema significantly (P 0.05) at a dose of 40mg/Kg, which was corroborated by paw volume and diameter, as well as haematological parameters, in Freund's complete adjuvant-induced arthritis model. The BCEE also significantly reduced pro-inflammatory cytokine levels and the histopathological changes caused by Freund's full adjuvant model. BCEE preserves synovial membranes by enhancing health and has shown a significant anti-arthritic activity. Thus, data confirms the traditional usage of Bombax ceiba for arthritis.
Keywords
Bombax ceiba, Freund’s complete adjuvant-induced arthritis, haematological, histopathological and organ wt.
Citation
Posinasetty et al. Bioinformation 19(8): 833-839 (2023)
Edited by
P Kangueane
ISSN
0973-2063
Publisher
License
This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.
