Title

Effects of vasicine in neuroinflammatory zebrafish model

Authors

Arthi Balasundaram^1,* & Darling Chellathai²

Affiliation

 ¹Department of Pharmacology, Sri Ramachandra Medical College and Research Institute, Sri Ramachandra Institute of Higher Education and Research, Porur, Chennai-600116, Tamil Nadu, India; ²Department of Pharmacology, Sri Ramachandra Medical College and Research Institute, Sri Ramachandra Institute of Higher Education and Research, Porur, Chennai-600116, Tamil Nadu, India; *Corresponding author

Email

Arthi Balasundaram - E - mail: rtms86@yahoo.com

Darling Chellathai - E - mail: daviddolly@rediff.com

Article Type

Research Article

Date

Received May 1, 2023; Revised May 31, 2023; Accepted May 31, 2023, Published May 31, 2023

Chronic neuroinflammation produces cytotoxic effects and aggravates neurodegeneration. Cognitive impairment was considered an early symptom of many neurodegenerative diseases. Therefore, it is of interest to evaluate the in-vivo efficacy of vasicine for treating neuroinflammation-induced cognitive impairments in the zebrafish model. The Neurobehavioral activity was evaluated with a dive tank test, swim motion test, plus maze test, turn angle test, and color preference test in three neuroinflammatory zebrafish models. Gene expression analysis was done for neuroinflammatory markers (IL-10, IL-15, IL-13, NogoA, Fetuin-A, BDNF, NAA, CXCL2, Osteopontin) using polymerase chain reaction and gel electrophoresis technique. Behavioral parameters and biochemical evaluations revealed that vasicine was effective in treatment against neuroinflammatory models of surgery, chemical, xenotransplantation. Attenuation of cognitive dysfunction in all three neuroinflammation zebrafish models by vasicine in this study may recommend vasicine as a potential molecule for treating neuroinflammatory and neurodegenerative diseases.

Keywords

Neuroinflammation, Neurodegeneration, Zebrafish, PCR, gel electrophoresis

Citation

Balasundaram & Chellathai, Bioinformation 19(5): 595-604 (2023)

Edited by

P Kangueane

ISSN

0973-2063

Publisher

Biomedical Informatics

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.