Title
SWATH-MS analysis of plasma proteins among Indian HIV-1 infected patients
Authors
Sushanta Kumar Barik1, Srikanth Prasad Tripathy2, Deepa Bisht1, Praveen Singh3, Rahul Chakraborty3, Shripad A Patil1, Tej Pal Singh4, Deepika Varshney1, Srikanta Jena5 & Keshar Kunja Mohanty1*
Affiliation
1ICMR-National JALMA Institute for Leprosy and Other Mycobacterial Diseases, Agra, Uttar-Pradesh, India-282004; 2ICMR-National Institute for Research in Tuberculosis, Chetpet, Chennai, Tamil Nadu, India-600031; 3CSIR-Institute of Genomics and Integrative Biology, CSIR, New Delhi-110025; 4Sarojini Naidu Medical College, Agra, Uttar-Pradesh, India-282003; 5Ravenshaw University, Odisha, Cuttack, India - 753003; *Corresponding author
Sushanta Kumar Barik - E-mail ID:
sushantakumarbarik82@gmail.com
Srikanth Prasad Tripathy - E-mail ID: srikanthtripathy@gmail.com
Deepa Bisht - E-mail ID: abd1109@rediffmail.com
Praveen Singh - E-mail ID: praveen05singh@gmail.com
Rahul Chakraborty - E-mail ID: rahul.c@igib.in
Shripad A Patil - E-mail ID: shripadpatil@yahoo.com
Tej Pal Singh - E-mail ID: drtejpal.singh@yahoo.com
Deepika Varshney - E-mail ID: deepika.biotech1@gmail.com
Srikanta Jena - E-mail ID: jenasrikanta@yahoo.co.in
Keshar Kunja Mohanty - E-mail ID: keshar63@yahoo.com
Article Type
Research Article
Date
Received April 1, 2023; Revised April 30, 2023; Accepted April 30, 2023, Published April 30, 2023
Abstract
The identification and characterization of plasma proteins in drug resistant and drug sensitive in HIV-1 infected/AIDS patients were carried out using the SWATH-MS protocol. In total, 204 proteins were identified and quantified, 57 proteins were differentially expressed, out of which 25 proteins were down regulated and 32 proteins were up regulated in drug resistant patients. Six proteins such as complement C4-A, immunoglobulin heavy variable 1-2, carboxylic ester hydrolase, fibulin-1, immunoglobulin lambda constant7, secreted phosphoprotein 24 were differentially expressed in individuals with drug resistant HIV as compared to individuals with drug sensitive HIV. Gene ontology of 57 differentially expressed proteins was analysed and documented.
Keywords
SWATH-MS, HIV-1, AIDS, ART, NRTI, NNRTI
Citation
Barik et al. Bioinformation 19(4): 392-398 (2023)
Edited by
P Kangueane
ISSN
0973-2063
Publisher
License
This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.
