Title
Anti-inflammatory activity of lauric acid, thiocolchicoside and thiocolchicoside-lauric acid formulation
Authors
Ameena Mustafa1, 2,*, Meignana Arumugham Indiran3, Rajeshkumar Shanmugham4 & Karthikeyan Ramalingam5
Affiliation
1Department of Oral Pathology and Microbiology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, Tamilnadu, India; 2Department of Oral Pathology and Microbiology, Azeezia College of Dental Sciences and Research, Meyyannoor, Kollam , Kerala, India; 3Department of Public Health Dentistry, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, Tamilnadu, India; 4Nanobiomedicine Lab, Department of Pharmacology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, Tamilnadu, India; 5Department of Oral Pathology and Microbiology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, Tamilnadu, India; *Corresponding author
Ameena Mustafa Email: 162103018.sdc@saveetha.com
Meignana Arumugham Indiran Email: drmei.sdc@saveetha.com
Rajeshkumar Shanmugham Email: rajeshkumars.sdc@saveetha.com
Karthikeyan Ramalingam Email: karthikeyanr.sdc@saveetha.com
Article Type
Research Article
Date
Received November 1, 2023; Revised November 30, 2023; Accepted November 30, 2023, Published November 30, 2023
Abstract
It is of interest to develop potent and safer anti-inflammatory drugs from plants, as medicinal plants and herbs attained great attention in the medical world due to their multifunctional activities. This article studied the anti-inflammatory effects of lauric acid (LA), thiocolchicoside (TC) and thiocolchicoside–lauric acid (TC-LA) formulation. The anti-inflammatory effects of these compounds were determined by following the methods of inhibition of protein denaturation and proteinase inhibition activity. This was assessed at different concentrations to determine the 50% inhibition concentration (IC50) of the compounds. The result indicated that the activity of LA, TC, TC-LA formulation, and reference drug increased with the increase in the concentration from 10-50 μg/ml, thus proving the activity of LA, TC, and TC-LA formulation against inflammation was in a dose-dependent manner. The percentage of inhibition of protein denaturation was 59.56%, 66.94%, 86.62%, and 60.34% for LA, TC, the combination of TC-LA and standard drug, and the IC50 values were found to be 44.78 μg/mL, 37.65 μg/mL, 27.15 μg/mL and 43.42 μg/mL, respectively. The percentage of proteinase inhibition activity of LA, TC, and a combination of TC-LA and the standard drug was 66.65%, 77.49%, 94.07%, and 69.83%, and IC50 of LA, TC, a combination of TC-LA and standard drug were35.5 μg/mL, 32.12 μg/mL, 24.35 μg/mL and 37.80 μg/mL, respectively. We found out that lauric acid, thiocolchicoside, and thiocolchicoside-lauric acid formulation exhibited significant anti-inflammatory activity.
Keywords
Lauric acid, thiocolchicoside, anti-inflammatory agent, spectrophotometer, dose dependant
Citation
Mustafa et al. Bioinformation 19(11): 1075-1080 (2023)
Edited by
P Kangueane
ISSN
0973-2063
Publisher
License
This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.
