Title

Effect of ß-Caryophyllene on insulin resistance in skeletal muscle of high fat diet and fructose-induced type-2 diabetic rats

Authors

Vadivel Mani¹, Ramya Badrachalam¹, Sharon Nallathannikulam Shanmugam², Manikandan
Balraj³, Revathi Kasthuri⁴, Anandhi Danavel^3,* & Shyamaladevi Babu⁵

Affiliation

¹Department of Biochemistry, Arunai Medical College & Hospital, Tiruvanamallai-606603, Tamilnadu, India; ²Department of biochemistry, MeenakshiAmmal Dental College & Hospital, Chennai-600095, Tamilnadu, India; ³Department of Physiology, Konaseema Institute of Medical sciences and research foundation, Amalapuram, East Godavari Dt-533201, Andhra Pradesh, India;
⁴Department of Research, Meenakshi Academy of Higher Education, Chennai-600078, Tamilnadu, India; ⁵Department of Biochemistry, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, Tamil Nadu, India

Email

^*Corresponding author; AnandhiDanavel – E-mail: thivyeshwar@gmail.com; Vadivel Mani-velvdmvel5@gmail.com; RamyaBadrachalam – drramya830@gmail.com; Sharon NallathannikulamShanmugamnorahs. jesus@gmail.com; ManikandanBalraj- drmani.ayurveda@gmail.com; RevathiKasthuri- reva63@rediffmail.com; Shyamaladevi Babu-shyamdevi06@gmail.com

Article Type

Research Article

Date

Received August 4, 2021; Revised August 30, 2021; Accepted August 30, 2021, Published August 31, 2021

High fat diet feeding results in hyperglycemia and insulin resistance, which is a major pathological feature of type-2 diabetes mellitus. The use of oral hypoglycaemic drugs is limited due to its deleterious side effects and there is a need to find more efficacious agents for diabetes management. Hence, it is of interest to show the mechanism of action of ß-Caryophyllene on insulin signalling molecules in gastrocnemius muscle of high fat diet- induced type-2 diabetic rats. An oral effective dose of with ß-Caryophyllene (200 mg/kg b.wt) was given for 30 days to high fat diet (comprising 2% cholesterol, 1% cholic acid, 30% coconut oil, 67% conventional rat feed) and fructose fed type-2 diabetic rats to find out whether ß-Caryophyllene regulates IRS-1/Akt pathway of insulin signalling. The data shows that, ß-Caryophyllene treatment significantly increased the mRNA and protein expression of insulin receptor (IR) in diabetic rats whereas there is no significant difference in mRNA expression of insulin receptor-substrate-1 (IRS-1) was observed among groups.
The Akt mRNAand GLUT-4mRNA and protein level were also improved in gastrocnemius muscle of type-2 diabetic rats. Thus, we concluded that ß–Caryophyllene could be used as potential phyto medicine for type-2 diabetes management.

Keywords

ß–Caryophyllene; High fat diet; Insulin resistance; Type-2 diabetes; IRS-1/Akt signaling; Glucose transporter

Citation

Mani et al. Bioinformation 17(8): 741-747 (2021)

Edited by

P Kangueane

ISSN

0973-2063

Publisher

Biomedical Informatics

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.