Title
Phylogenetic and modelling analysis of purple acid phosphatase 18 (SiPAP18) from Setaria italica
Authors
Chinreddy Subramanyam Reddy1,*, Tanushri Kaul2,*, Khaled Fathy Abdel Motelb1, Sonia Khan Sony1, Jyotsna Bharti1 & Rachana Verma1
Affiliation
1Department of Botany, CSSR and SRRM Degree College, Kamalapuram, Kadapa District, Andhra Pradesh-516219, India; 2Nutritional Improvement of Crops Group, International Center for Genetic Engineering and Biotechnology, New Delhi, India.
*Corresponding authors: Chinreddy Subramanyam Reddy - E-mail: suumani@gmail.com; Tanushri Kaul - E-mail: tanushri@icgeb.res.in;kaultanushri3@gmail.com
Article Type
Research Article
Date
Received July 23, 2021; Revised August 30, 2021; Accepted August 30, 2021, Published August 31, 2021
Abstract
Purple acid phosphatases belong to metallo-phosphatase family. Intracellular phosphatases are crucial for phosphorus (P) distribution in the cell and are highly induced in phosphorus-deprived conditions in the soil. Disparate PAP isoforms exist within discrete subcellular compartments in Setaria italica and their expression in P deprived conditions fosters phosphorus amelioration. We isolated the SiPAP18 gene and developed the homology SiPAP18 protein model based on the crystal structure of the Kidney bean PvPAP (PDB ID: 2QFP) as template (sequence similarity 42.7%) using Modeller 9.12 with adequate validation. Structure model analysis shows the significance of five conserved signatures with seven metal-paired amino acid residues during P-deprivation induced phosphorus amelioration.
Keywords
Purple acid phosphatase, protein modelling, phylogenetic analysis, phosphorus accessibility
Citation
Reddy et al. Bioinformation 17(8): 727-730 (2021)
Edited by
P Kangueane
ISSN
0973-2063
Publisher
License
This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.
