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Title

MTT assay based inhibition analysis of A2780 cells proliferation using Mollugo nudicaulis Lam. extract with CXCR4 and HER2 expression

 

Authors

Chella Perumal Palanisamy1,*, Shazia Fathima JH2, Ramya Sekar3, Ponnulakshmi Rajagopal4 & Selvaraj Jeyaraman5*

 

Affiliation

1State Key Laboratory of Biobased Material and Green Papermaking, School of Food Science and Engineering, Qilu University of Technology, Shandong Academy of Science, Jinan 250353, China;  2Department of Oral and Maxillofacial Pathology, Ragas Dental College and Hospitals, Chennai, India; 3Department Oral Pathology, Meenakshi Ammal Dental College, Chennai-600 095, India; 4Central Research Laboratory, Meenakshi Academy of Higher Education and Research (Deemed to be University), Chennai-600 078, India; 5Department of Biochemistry, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai - 600 077, India. *Corresponding authors;

 

Email

Dr. Selvaraj Jeyaraman - Email:jselvaendo@gmail.com; perumalbioinfo@gmail.com;

 

Article Type

Research Article

 

Date

Received May 5, 2021; Revised July 19, 2021; Accepted July 19, 2021, Published July 31, 2021

 

Abstract

It is of interest to document the inhibition of A2780 cell proliferation using Mollugo nudicaulis Lam. (M. nudicaulis) extract by MTT assay and by monitoring the CXCR4 and HER2 expression through RT-PCR analysis. Results shown that the n-hexane extract of M. nudicaulis have anticancer activity IC50 values of 32.46±0.92 Ĵg/mL on A2780 cell lines. It is further found that the CXCR4 and HER2
mRNA and protein expression were significantly reduced in M. nudicaulis treated A2780 cell lines. Thus, the n-hexane extract of M. nudicaulis is a natural source of bioactive compounds as potential anticancer agents.

 

Keywords

M. nudicaulis; n-hexane extract; CXCR4 and HER2 protein expression, Metastatic signalling, Anti-ovarian cancer activity

 

Citation

Palanisamy et al. Bioinformation 17(7): 705-709 (2021)

 

Edited by

P Kangueane

 

ISSN

0973-2063

 

Publisher

Biomedical Informatics

 

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.