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Title |
In vitro cytotoxicity
analysis of Zizyphus spina-christi stem bark extract on human cancer
cell lines
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Authors |
Saravanan Rajendrasozhan1*, Essam N. Ads2, Amr S. Abouzied3,4 &
Jamal R. Humaidi1 |
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Affiliation |
1Department of Chemistry, College of Sciences, University of Ha!il,
Ha!il 55476, Kingdom of Saudi Arabia; 2Faculty of Science, Zagazig
University, El Mansoura 35522, Egypt; 3Department of Pharmaceutical
Chemistry, College of Pharmacy, University of Ha!il, Ha!il 55476,
Kingdom of Saudi Arabia; 4Department of Pharmaceutical Chemistry,
National Organization for Drug Control and Research, Giza 35521,
Egypt. |
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Email |
Saravanan Rajendrasozhan - Email: s.rajendrasozhan@uoh.edu.sa; Essam
N. Ads - Email: essam.adss@yahoo.com; Amr S. Abouzied - Email:
as.ibrahim@uoh.edu.sa; Jamal R. Humaidi - Email: j.humaidi@uoh.edu.sa |
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Article Type |
Research Article
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Date |
Received April 29, 2021;
Revised May 29, 2021; Accepted May 29, 2021, Published May 31, 2021
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Abstract |
Zizyphus spina-christi (Rhamnaceae family) is an edible plant used
in folk medicine. Therefore, it is of interest to report the
cytotoxic effects of Z. spina-christi bark crude extract on human
cell lines. Crude ethanol extract of Z. spina-christi bark was
fractionated with increasing polarity (diethyl ether, chloroform,
ethyl acetate and butanol fractions). The fractions were examined
for their cytotoxicity against human colon cancer (HCT-116 and
CACO-2), cervical cancer (HeLa and HEp-2), lung carcinoma (A-549),
hepatocellular carcinoma (HepG-2), breast cancer (MCF-7) and
prostate cancer (PC-3) cell lines using viability assay. Diethyl
ether fraction of Z. spina-christi showed the highest cytotoxic
effects among the four extracts of Z. spina-christi. The IC50 of
diethyl ether fraction was 7.14, 11.2, 11.6, 15.4, 39.8, 42.2, 84.2
and 153.8 Ĵg/ml on HepG-2, A-549, CACO-2, HCT-116, MCF-7, PC-3, HeLa,
and HEp-2 cell lines, respectively. Data shows that the diethyl
ether fraction of Z. spina-christi showed effective cytotoxic
effects in colon, lung and hepatocellular cancer cell lines. |
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Keywords |
Anticancer; cytotoxic; phytochemicals; natural remedies, Zizyphus
spina-christi |
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Citation |
Rajendrasozhan et al.
Bioinformation 17(5): 583-592 (2021) |
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Edited by |
P Kangueane
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ISSN
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0973-2063
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Publisher |
Biomedical Informatics
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License |
This is an Open Access article which permits unrestricted use,
distribution, and reproduction in any medium, provided the original
work is properly credited. This is distributed under the terms of
the
Creative Commons Attribution License.
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