Title

Transcriptome wide identification and characterization of Starch Synthase enzyme in finger millet

Authors

Rajhans Tyagi¹, Apoorv Tiwari^2,3, Alok Kumar Gupta⁴, Sanjay Gupta⁵

Affiliation

¹Uttarakhand Technical University, Dehradun, Uttarakhand, 248007, India;

²Sam Higginbottom University of Agriculture, Technology and Sciences (SHUATS), Allahabad, 211007, India;

³Dept of Molecular Biology and Genetic Engineering, G.B. Pant University of Agriculture and Technology, Pantnagar, Uttarakhand, 263145, India;

⁴Amity University Uttar Pradesh, Noida. 201313, India;

⁵Himalayan School of Biosciences, Swami Rama Himalayan University, Jolly Grant, Dehradun, 248016, India

Email

rajhans.gaheli@gmail.com

Article Type

Hypothesis

Date

Received July 7, 2018; Revised July 10, 2018; Accepted July 30, 2018; Published July 31, 2018

Finger millet is a calcium-rich cereal crop of the grass family. The transcriptome data for finger millet is available at NCBI. It is of interest to annotate and characterize starch synthase enzyme from finger millet transcriptome data. Starch synthase plays an important role in the elongation of glucan chains during the formation of starch. The starch synthase enzyme is characterized using three domains (Glyco_transf_5, Glycos_transf_1 and Glyco_trans_1_4). Binding sites for GLC (alpha-d-glucose), PLP (Pyridoxal-5'- phosphate), AMP (Adenosine monophosphate) and GOL (Glycerol) are found. The phylogenetic analysis showed that the finger millet starch synthase is similar to the granule-bound starch synthase of Oryza sativa and Concrete amaricanus. We report the sequence (GenBank accession number KY648917) and the structural model of finger millet starch synthase (PMDB ID: PM0081600).

Keywords

Finger millet, CDS, SBE, Domain, PMDB, NCBI

Citation

Tyagi et al. Bioinformation 14(7): 393-397 (2018)

Edited by

P Kangueane

ISSN

0973-2063

Publisher

Biomedical Informatics

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.