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Title |
Identification of coding sequence and its use for functional and structural characterization of catalase from Phyllanthus emblica
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Authors |
Swati Sharma, Vinita Hooda*
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Affiliation |
1Department of Botany, Faculty of Life Sciences, Maharshi Dayanand University, Rohtak-124001, India
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vinitahooda.botany@mdurohtak.ac.in
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Article Type |
Hypothesis
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Date |
Received December 21, 2017; Revised December 31, 2017; Accepted January 16, 2018; Published January 31, 2018
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Abstract |
Catalase is an essential antioxidant enzyme that is well characterized from microbial and animal sources. The structure of plant catalase is unknown. Therefore, it is of interest to understand the functional and structural characteristics of catalase from an Indian gooseberry, Phyllanthus emblica (or Emblica officinalis). Hence, catalase from P. emblica was cloned in pUC18 plasmid, sequenced and submitted to GenBank with the accession numbers “MF979112” and “ATO98311.1. InterProScan showed that the coding sequence is monofunctional and haem-dependent catalase-like superfamily. Multiple sequence alignment (MSA) followed by phylogenetic analysis showed that the P. emblica catalase groups with soybean catalase. We further report the characteristics of structural model of the enzyme for functional characterization.
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Keywords |
Catalase, Antioxidant enzyme, Phyllanthus emblica, GenBank, InterProScan, Multiple sequence alignment, Phylogenetic analysis
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Citation |
Sharma & Hooda. Bioinformation 14(1): 008-014 (2018)
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Edited by |
P Kangueane
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ISSN |
0973-2063
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Publisher |
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License |
This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.
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