Title

Structural modelling and phylogenetic analyses of PgeIF4A2 (Eukaryotic translation initiation factor) from Pennisetum glaucum reveal signature motifs with a role in stress tolerance and development

Authors

Aakrati Agarwal1,2, Yashwanti Mudgil,2 Saurabh Pandey1, Dhirendra Fartyal1, and Malireddy K Reddy1*

Affiliation

1Plant Molecular Biology Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi- 110067, India; 2Plant Molecular Biology Lab, Department of Botany, University of Delhi, New Delhi, India;

Email

Dr. M. K. Reddy - Phone: +91-11-26742962; Fax: +91-11-26742316; *Corresponding Author

Article Type

Hypothesis

Date

Received August 2, 2016; Revised October 27, 2016; Accepted October 28, 2016; Published December 19, 2016

Eukaryotic translation initiation factor 4A (eIF4A) is an indispensable component of the translation machinery and also play a role in developmental processes and stress alleviation in plants and animals. Different eIF4A isoforms are present in the cytosol of the cell, namely, eIF4A1, eIF4A2, and eIF4A3 and their expression is tightly regulated in cap-dependent translation. We revealed the structural model of PgeIF4A2 protein using the crystal structure of Homo sapiens eIF4A3 (PDB ID: 2J0S) as template by Modeller 9.12. The resultant PgeIF4A2 model structure was refined by PROCHECK, ProSA, Verify3D and RMSD that showed the model structure is reliable with 77 % amino acid sequence identity with template. Investigation revealed two conserved signatures for ATP-dependent RNA Helicase DEAD-box conserved site (VLDEADEML) and RNA helicase DEAD-box type, Q-motif in sheet-turn-helix and α-helical
region respectively. All these conserved motifs are responsible for response during developmental stages and stress tolerance in plants.

Keywords

eIF4A2, Eukaryotic initiation factor, helicase, structural modelling, Pennisetum glaucum

Citation

Agarwal et al. Bioinformation 12(12): 416-419 (2016)

Edited by

P Kangueane

ISSN

0973-2063

Publisher

Biomedical Informatics

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.