Title

Identification and characterization of conserved miRNAs with its targets mRNA in Trichinella Spiralis

Authors

Dyavegowda Padmashree & Narayanaswamy Ramachandraswamy*

Affiliation

Department of Biochemistry, Central College Campus, Bangalore University, Bangalore, Karnataka, India;

Email

Narayanaswamy Ramachandraswamy - E-mail: drnrswamy@bub.ernet.in; Corresponding author; Telephone number: +91 080 22961335

Article Type

Hypothesis

Date

Received June 16, 2016; Revised July 10, 2016; Accepted July 11, 2016; Published August 15, 2016

microRNAs (Small regulatory non-coding RNAs) have an important role in gene regulation and evolutionarily conserved molecules. Trichinella spiralis infect majority of species. Therefore, it is of interest to identify conserved miRNAs and their targets using sequences from EST, GSS and full length nucleotides obtained from NCBI against previously reported worm miRNAs. We identify 11 novel
miRNAs in T. spiralis by using bioinformatics-homology based search. In addition, we predicted target mRNA genes form complementary base pair in seed region of miRNAs. Further, gene annotation using Uniprot shows that these target genes of miRNAs are involved in various metabolism, enzymatic activity and constituents of membrane components.

Keywords

miRNA, computational, T. spiralis, prediction, annotation

Citation

Padmashree & Ramachandraswamy, Bioinformation 2016 12(5) 279-284.

Edited by

P Kangueane

ISSN

0973-2063

Publisher

Biomedical Informatics

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.