Title

Insights using the molecular model of Lipoxygenase from Finger millet (Eleusine coracana (L.))

Authors

Apoorv Tiwari1,2,#, Himanshu Avashthi1,2,#, Richa Jha5,#, Ambuj Srivastava4,#, Vijay Kumar Garg1,2,#, Pramod Wasudev Ramteke3,# and Anil Kumar1,#*

Affiliation

1Department of Molecular Biology & Genetic Engineering, CBSH, G. B. Pant University of Agriculture & Technology, Pantnagar-263145, Uttarakhand, Bharat (India). 2Department of Computational Biology & Bioinformatics, JSBB, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad-211007, Uttar Pradesh, Bharat (India). 3Department of Biological Science, School of Basic Science, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad-211007, Uttar Pradesh, Bharat (India). 4Department of Biotechnology, Indian Institute of Technology Madras, Chennai-600036, Tamil Nadu, Bharat (India). 5Uttaranchal Institute of Technology,
Uttaranchal University, Arcadia Grant, Dehradun-248007, Uttarakhand Bharat (India).

Email

Pramod Wasudev Ramteke – E-mail: pwramteke@yahoo.com; Prof. Anil Kumar - Email: anilkumar.mbge@gmail.com; *Corresponding author; #Equal contribution

Article Type

Hypothesis

Date

Received January 30, 2016; Revised May 17, 2016; Accepted May 18, 2016; Published June 15, 2016

Lipoxygenase-1 (LOX-1) protein provides defense against pests and pathogens and its presence have been positively correlated with plant resistance against pathogens. Linoleate is a known substrate of lipoxygenase and it induces necrosis leading to the accumulation of isoflavonoid phytoalexins in plant leaves. Therefore, it is of interest to study the structural features of LOX-1 from Finger millet. However, the structure ofLOX-1 from Finger millet is not yet known. A homology model of LOX-1 from Finger millet is described. Domain architecture study suggested the presence of two domains namely PLAT (Phospho Lipid Acyl Transferase) and lipoxygenase. Molecular docking models of linoleate with lipoxygenase from finger millet, rice and sorghum are reported. The features of docked models showed that finger millet have higher pathogen resistance in comparison to other cereal crops. This data is useful for the molecular cloning of full length LOX-1 gene for validating its role in improving plant defense against pathogen infection and for various other biological processes.

Keywords

Lipoxygenase, Eleusine coracana, pest resistance, homology modeling, Structure prediction, docking, protein-protein interaction.

Citation

Tiwari et al. Bioinformation 12(3): 156-164 (2016)

Edited by

P Kangueane

ISSN

0973-2063

Publisher

Biomedical Informatics

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.