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Title

MFPPI Multi FASTA ProtParam Interface

 

Authors

Vijay Kumar Garg1, 2, Himanshu Avashthi1, Apoorv Tiwari1, Prashant Ankur Jain1 Pramod
Wasudev Ramkete3*, Arvind Mohan Kayastha2 &Vinay Kumar Singh2

 

Affiliation

1Department of Computational Biology & Bioinformatics, Jacob School of Biotechnology & Bioengineering, Sam Higginbottom Institute of Agriculture Technology & Sciences, Allahabad-211007, Uttar Pradesh, Bharat (India); 2Centre for Bioinformatics, School of Biotechnology, Institute of Science, Banaras Hindu University, Varanasi-221005, Uttar Pradesh, Bharat (India); 3Department of Biological Sciences, Sam Higginbottom Institute of Agriculture Technology & Sciences, Allahabad-211007, Uttar Pradesh, Bharat
India;

 

Email

Pramod Wasudev Ramkete Email: pwramteke@yahoo.com; Vinay Kumar Singh Email: vinaysingh@bhu.ac.in; Phone No.0542-2368364; *Corresponding author

 

Article Type

Web Server

 

Date

Received January 30, 2016; Revised March 10 2016; Accepted March 12, 2016; Published April 10, 2016

Abstract

Physico-chemical properties reflect the functional and structural characteristics of a protein. The comparative study of the physicochemical properties is important to know role of a protein in exploring its molecular evolution. A number of online and offline tools are available for calculating the physico-chemical properties of a single protein sequence. However, a tool is not available for
a comparative study with graphical visualization of Multi-FASTA sequences. Hence, we describe the development and utility of MFPPI V.1.0 (a web interface developed in JAVA platform) to input each FASTA sequence from Multi-FASTA file into the ProtParam web server for the calculation of physico-chemical properties. MFPPI V.1.0 calculates different physico-chemical properties for a given set of proteins in a single run and saves the data in the MSExcel sheet. Furthermore, it provides a graphical representation of protein physico-chemical properties for analysis and visualization of data in a user-friendly manner. Therefore, the output from the analysis helps to understand compositional changes and functional relationship in evolution among organisms. We have demonstrated the utility of MFPPI V.1.0 using 17 mtATP6 protein sequences from different mammalian species. It is available for free at http://insilicogenomics.in/mfpcalc/mfppi.html.

 

Keywords

Physico-chemical Property, Multi-FASTA Proteins, Amino acid richness, Peptide hydrophobicity, Isoelectric point and Extinction coefficient.

 

Citation

Garg et al. Bioinformation 12(2): 74-77 (2016)
 

Edited by

P Kangueane

 

ISSN

0973-2063

 

Publisher

Biomedical Informatics

 

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.