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Title |
Molecular model of thylakoid membrane bound (SlAPX6) ascorbate peroxidase from Solanum lycopersicum
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Authors |
Kaushlendra Tripathi1, 2, Saurabh Pandey1, Murtaza Malik1, 2, Krishnamurthy Sathelly, Tanushri Kaul1
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Affiliation |
1Nutritional Crop Improvement Group,
International Centre for Genetic Engineering and Biotechnology,
Aruna Asaf Ali Marg, New Delhi-110067, India; 2Department of
Biotechnology, Shri Venkateshwara University Venkateshwara Nagar,
Rajabpur
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Tanushri Kaul Email: tanushrikaul@gmail.com; tanushri@icgeb.res.in;
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Article Type |
Hypothesis
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Date |
Received February 15, 2016; Revised March 02, 2016; Accepted March 03, 2016; Published April 10, 2016
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Abstract |
Ascorbate peroxidase (E 1.11.1.11) acts as primary key component of plant defense against photo protection and photo-oxidative stress. Chloroplastic (APX) located in the thylakoid membrane (tAPX) and stroma (sAPX) have been thought to be key regulators of intracellular levels of H2O2. Therefore, it is of interest to study thylakoid membrane bound SlAPX from Solanum lycopersicum (tomato, a fleshy fruit). However, a structure model is not yet solved for tomato thylakoid membrane bound SlAPX. “Hence, a homology molecular model of SlAPX6 from S. lycopersicum was constructed using a template structure (PDB ID: 1APX) from Pisum sativum. The model was further assessed using accessible surface area (ASA) calculations to identify surface residues for further characterization of active site regions. We further characterized the active site regions in the enzyme for functional inference. This information provides insights for the understanding of photo protection and photo-oxidative stress tolerant in S. lycopersicum during flower development and fruit ripening.”
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Keywords |
Ascorbate peroxidase, Solanum lycopersicum, Pisum sativum |
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Citation |
Tripathi et al. Bioinformation 12(2):
44-47 (2016) |
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Edited by |
P Kangueane
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ISSN |
0973-2063
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Publisher |
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License |
This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License. |