Title

Antimicrobial effect of Pistacia atlantica leaf extract

Authors

Affiliation

¹Department of Perio-dentistry, Faculty of Dentistry, Ilam University of Medical Sciences,  Iran

²Department Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Iran

³Mostafa Khomeini Hospital, Ilam University of Medical Sciences, Iran

⁴Department Microbiology, Faculty of Medicine, Ilam University of Medical Sciences, Iran

⁵Clinical Microbiology Research Center, Ilam University of Medical Sciences, Iran

Email

mpvmpv559@gmail.com;   *Corresponding author

Article Type

Hypothesis

Date

Received January 05, 2016; Accepted January 07, 2016; Published January 31, 2016

The antimicrobial effect of the mastic tree (Pistacia atlantica) under in vitro conditions has been reported. Therefore, it is of interest to evaluate the effect of the plant leaf extract (aqueous) on bacterial load in mouth and saliva. The leaf of the Pistacia atlantica plant was collected and cleaned, dried at 40⁰c and then powdered. The extraction was carried out using the maceration method in vacuum with the rotary evaporator device. Bacterial inhibition (Streptococcus species) by the leaf extract was studied using the disc diffusion and embedding sink diffusion methods. The values of MIC and MBC were determined. The collected data was further analyzed using t-test and repeated measure statistical tests. The disc diffusion technique showed a significant inhibitory effect for Pistacia atlantica’s leaf extract on S. mutans (ATCC 35668) and S. mitis (ATCC 49456) with inhibition zones of 19 and 25 millimeters, respectively. This is for the highest leaf extract concentration used in this study (p<0.01). The values of MIC and MBC for S. mutans was 60, 90 μg/ml and for S. mitis was 75, 110 μg/ml (p<0.01 significance). The leaf extract has no significant effect on S. salivarius (ATCC 13419). Thus, the antimicrobial properties of the aqueous leaf extract from Pistacia atlantica is demonstrated in this study.

Pistacia atlantica, leaf extract, anti-bacterial, Streptococcus mutans, Streptococcus mitis, Streptococcus salivarius

Citation

Roozegar et al. Bioinformation 12(1): 19-21 (2016)
 

Edited by

P Kangueane

ISSN

0973-2063

Publisher

Biomedical Informatics

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.