Interaction pattern for the complex of B-DNA-Fullerene compounds with a set of known replication proteins using docking study

BACK TO CONTENTS | PDF | PREVIOUS | NEXT

Title	Interaction pattern for the complex of B-DNA-Fullerene compounds with a set of known replication proteins using docking study
Authors	Sumbul Firdaus¹, Anupam Dhasmana², Vandana Srivastava², Tasneem Bano², Afreen Fatima², Qazi Mohammad Sajid Jamal³, Roshan Jahan³, Gulshan Wadhwad⁴ & Mohtashim Lohani³*
Affiliation	¹Department of Physics, Integral University, Lucknow, India; ²Departments of Bioengineering, Integral University, Lucknow, India; ³Departments of Biosciences, Integral University, Lucknow, India; ⁴Department of Biotechnology, Ministry of Science and Technology, CGO complex, Lodhi Road, New Delhi-110003, India
Email	mlohani@rediffmail.com; *Corresponding author
Article Type	Hypothesis
Date	Received January 21, 2015; Accepted February 23, 2015; Published March 31, 2015
Abstract	Fullerenes have attracted considerable attention due to their unique chemical structure and potential applications which has opened wide venues for possible human exposure to various fullerene types. Therefore, in depth knowledge of how fullerene may interfere with various cellular processes becomes quite imperative. The present study was designed to investigate how the presence of fullerene affect the binding of DNA with different enzymes involved in replication process. Different fullerenes were first docked with DNA and then binding scores of different enzymes was analyzed with fullerene docked DNA. C30, C40 & C50 once docked with DNA, reduced the binding score of primase, whereas no significant change in the binding score was observed with the helicase, ssb protein, dna pol δ, dna pol ɛ, ligase, DNA clamp, and topoisomerases. On the contrast, the binding score of RPA14 decreases in fluctuating manner while interacting with increasing molecular weight of fullerene bound single-stranded DNA complex. The study revealed the affect of fullerene family interacting with DNA on the binding pattern of enzymes involved in replication process. Study suggests that the presence of most of fullerenes may not affect the activity of these enzymes necessary for replication process whereas C30, C40 & C50 may disrupt the activity of primase, (strating point for DNA polymerase) its docking score decreases from 13820 to 10702.
Keywords	Fullerene, Fullerene family, RPA, replication enzymes.
Citation	Firdaus et al. Bioinformation 11(3): 122-126 (2015)
Edited by	P Kangueane
ISSN	0973-2063
Publisher	Biomedical Informatics
License	This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.