Title

Authors

Affiliation

¹Department of Urology, King George’s Medical University, Lucknow 226003, Uttar Predesh, India; ²Department of Microbiology, King George’s Medical University, Lucknow 226003, Uttar Predesh, India; ³Metal Analysis Division, (CSIR)-Indian Institute of Toxicology Research, Post Box, No. 80, M.G. Marg, Lucknow 226001, Uttar Pradesh, India

Email

singhvishwa69@gmail.com; *Corresponding author

Article Type

Hypothesis

Date

Received November 07, 2014; Accepted November 12, 2014; Published December 31, 2014

The induced toxicity of three pesticides (alpha-Hexachlorocyclohexane: ɑ-HCH; Parathion methyl:PM; Carbofuran: CN) in single and four possible combination on human keratinocyte cell line have been investigated. There was no significant change in toxicity (cyto and genotoxicity) on cell line exposed by individual pesticides except ɑ-HCH. But, a synergistic effect was observed when we tested mixture of pesticides. The intracellular ROS and cytotoxicity assay revealed maximum reduction in cell viability (60%) was found in tri mixture of pesticides. All the possible combination of these pesticides demonstrated genotoxic activity in terms of olive tail moment and % tail DNA on cell line at low concentration. The order of toxicity was ranked as ɑ-HCH+PM+CN>ɑ-HCH+CN>PM+CN>ɑ-HCH+PM. Our results call for more research to be undertaken in order to understand the mechanisms behind the synergy observed and quantify the extent of its environmental impacts.

Keywords

In-vitro, Toxicity, Genotoxicity, Pesticides, Cell line.

Citation

Abhishek et al.   Bioinformation 10(12): 716-720 (2014)
 

Edited by

P Kangueane

ISSN

0973-2063

Publisher

Biomedical Informatics

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.