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Title

Study of the inhibitory effect of Quercus Coccifera's aqueous extract on Staphylococcus aureus and Pseudomonas aeruginosa In vitro

 

Authors

Arezo Judaki1, Jafar Panahi2, Mohamad Reza Havasian2, Parnian Tajbakhsh2, Mohamad Ali Roozegar3*

 

Affiliation

1Department of Gastroenterology, Ilam University of Medical Sciences, Ilam/ Iran; 2Student research of committee, Ilam University of Medical Sciences, Ilam/Iran; 3Department of Periodontics, Faculty of Dentistry, Ilam University of Medical Sciences, Ilam, Iran

 

Email

pugpub29@gmail.com; *Corresponding author

 

Article Type

Hypothesis

 

Date

Received November 15, 2014; Accepted November 18, 2014; Published November 27, 2014

 

Abstract

The use of therapeutic herbs has become of great importance these days due to the increase in drug resistance. From a long time ago the Venus' navel plant has been used to treat infections. In this study the antibacterial effect of the aqueous extract from the Quercus coccifera (jaft) herb, under laboratory conditions. This study was carried out experimentally. After collecting the Venus navel herb, it was dried in a warm dry environment away from direct sunlight in the shade. The alcoholic extract was prepared using a standard method. Clinical samples of staphylococcus aureus and pseudomonas aeruginosa were acquired from Ilam's health care institutes. The inhibitory effect of the extracts was analysed in the Mueller Hinton using the disk diffusion method for both bacteria. Then MIC and MBC of the extracts was determined using the Macro broth dilution method. At its highest concentration the aqueous extract had an inhibition zone of 27.2 and 23.7 mm on staphylococcus aureus and pseudomonas aeruginosa consecutively. The MIC and MBC for staphylococcus aureus were 10 and 12.5 μg/ml and for pseudomonas aeruginosa they were 10 and 17.5 μg/m consecutively. The results of this study show the strong antimicrobial effect of jaft's aqueous extract on staphylococcus aureus and pseudomonas aeruginosa and if more studies are based on this topic it could be a substitute for common antibiotics.

 

Keywords

aqueous extract, staphylococcus aureus, pseudomonas aeruginosa, MIC, MBC.

 

Citation

Judaki et al.   Bioinformation 10(11): 689-692 (2014)
 

Edited by

P Kangueane

 

ISSN

0973-2063

 

Publisher

Biomedical Informatics

 

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.