Phylogenetic and Comparative Sequence Analysis of Thermostable Alpha Amylases of kingdom Archea, Prokaryotes and Eukaryotes

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Title	Phylogenetic and Comparative Sequence Analysis of Thermostable Alpha Amylases of kingdom Archea, Prokaryotes and Eukaryotes
Authors	Tayyaba Huma¹*, Arooma Maryam¹, Shahid ur Rehman², Muhammad Tahir ul qamar¹, Tayyaba Shaheen¹, Asma Haque¹ &Bushra Shaheen¹
Affiliation	¹Department of Bioinformatics and Biotechnology, Government College University (GCUF), 38000, Faisalabad, Punjab, Pakistan; ²Department of Poultry Sciences, University of Agriculture (UAF), 38000, Faisalabad, Punjab, Pakistan
Email	tayyabashahbaz@gmail.com; *Corresponding author
Article Type	Hypothesis
Date	Received June 26, 2014; Accepted June 27, 2014; Published July 22, 2014
Abstract	Alpha amylase family is generally defined as a group of enzymes that can hydrolyse and transglycosylase α-(1, 4) or α-(1, 6) glycosidic bonds along with the preservation of anomeric configuration. For the comparative analysis of alpha amylase family, nucleotide sequences of seven thermo stable organisms of Kingdom Archea i.e. Pyrococcus furiosus (100-105慢), Kingdom Prokaryotes i.e. Bacillus licheniformis (90-95慢), Geobacillus stearothermophilus (75慢), Bacillus amyloliquefaciens (72慢), Bacillus subtilis (70慢) and Bacillus KSM K38 (55慢) and Eukaryotes i.e. Aspergillus oryzae (60慢) were selected from NCBI. Primary structure composition analysis and Conserved sequence analysis were conducted through Bio Edit tools. Results from BioEdit shown only three conserved regions of base pairs and least similarity in MSA of the above mentioned alpha amylases. In Mega 5.1 Phylogeny of thermo stable alpha amylases of Kingdom Archea, Prokaryotes and Eukaryote was handled by Neighbor-Joining (NJ) algorithm. Mega 5.1 phylogenetic results suggested that alpha amylases of thermo stable organisms i.e. Pyrococcus furiosus (100-105慢), Bacillus licheniformis (90-95慢), Geobacillus stearothermophilus (75慢) and Bacillus amyloliquefaciens (72慢) are more distantly related as compared to less thermo stable organisms. By keeping in mind the characteristics of most thermo stable alpha amylases novel and improved features can be introduced in less thermo stable alpha amylases so that they become more thermo tolerant and productive for industry.
Keywords	Alpha amylase, Kingdom archea, Thermo stable, Phylogenetic analysis
Citation	*Huma et al.* Bioinformation 10(7): 443-448 (2014)
Edited by	P Kangueane
ISSN	0973-2063
Publisher	Biomedical Informatics
License	This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.