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Title

Phylogenetic and Comparative Sequence Analysis of Thermostable Alpha Amylases of kingdom Archea, Prokaryotes and Eukaryotes

 

Authors

Tayyaba Huma1*, Arooma Maryam1, Shahid ur Rehman2, Muhammad Tahir ul qamar1, Tayyaba Shaheen1, Asma Haque1 &Bushra Shaheen1

 

Affiliation

1Department of Bioinformatics and Biotechnology, Government College University (GCUF), 38000, Faisalabad, Punjab, Pakistan; 2Department of Poultry Sciences, University of Agriculture (UAF), 38000, Faisalabad, Punjab, Pakistan

 

Email

tayyabashahbaz@gmail.com; *Corresponding author

 

Article Type

Hypothesis

Date

Received June 26, 2014; Accepted June 27, 2014; Published July 22, 2014

 

Abstract

Alpha amylase family is generally defined as a group of enzymes that can hydrolyse and transglycosylase α-(1, 4) or α-(1, 6) glycosidic bonds along with the preservation of anomeric configuration. For the comparative analysis of alpha amylase family, nucleotide sequences of seven thermo stable organisms of Kingdom Archea i.e. Pyrococcus furiosus (100-105ºC), Kingdom Prokaryotes i.e. Bacillus licheniformis (90-95ºC), Geobacillus stearothermophilus (75ºC), Bacillus amyloliquefaciens (72ºC), Bacillus subtilis (70ºC) and Bacillus KSM K38 (55ºC) and Eukaryotes i.e. Aspergillus oryzae (60ºC) were selected from NCBI. Primary structure composition analysis and Conserved sequence analysis were conducted through Bio Edit tools. Results from BioEdit shown only three conserved regions of base pairs and least similarity in MSA of the above mentioned alpha amylases. In Mega 5.1 Phylogeny of thermo stable alpha amylases of Kingdom Archea, Prokaryotes and Eukaryote was handled by Neighbor-Joining (NJ) algorithm. Mega 5.1 phylogenetic results suggested that alpha amylases of thermo stable organisms i.e. Pyrococcus furiosus (100-105ºC), Bacillus licheniformis (90-95ºC), Geobacillus stearothermophilus (75ºC) and Bacillus amyloliquefaciens (72ºC) are more distantly related as compared to less thermo stable organisms. By keeping in mind the characteristics of most thermo stable alpha amylases novel and improved features can be introduced in less thermo stable alpha amylases so that they become more thermo tolerant and productive for industry.

 

Keywords

Alpha amylase, Kingdom archea, Thermo stable, Phylogenetic analysis

 

Citation

Huma  et al. Bioinformation 10(7): 443-448 (2014)
 

Edited by

P Kangueane

 

ISSN

0973-2063

 

Publisher

Biomedical Informatics

 

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.