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Title |
Study of rust resistance genes in wheat germplasm with DNA markers
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Authors |
Asma Haque1, Tayyaba Shaheen1*, Tahsin Gulzar2, Mahmood ur Rahman1, Fatima Jalal1, Summera Sattar1, Beenish Ehsan1, Zafar Iqbal3 & Muhammad Younas3 |
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Affiliation |
1Department of Bioinformatics and Biotechnology, Government College University, Faisalabad; 2Department of Applied Chemistry, Government College University, Faisalabad; 3Agriculture Biotechnology Research Institute, AARI, Faisalabad
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tayaba_pgmb@yahoo.com; *Corresponding author
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Article Type |
Hypothesis |
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Date |
Received May 04, 2014; Accepted May 04, 2014; Published June 30, 2014
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Abstract |
Wheat is a vital dietary component for human health and widely consumed in the world. Wheat rusts are dangerous pathogens and contribute serious threat to its production. In present study, PCR-Based DNA Markers were employed to check the rust resistance genes among 20 wheat genotypes and 22 markers were amplified. NTSYS-pc 2.2 was used to calculate genetic diversity and Nei and Li's coefficients ranged from 0.55 to 0.95. Cluster analysis was obtained using UPGMA (Unweighted Pair Group Method of Arithmetic Average) algorithm. Maximum no. of genes (23) was amplified from TW-760010 genotype whereas minimum no of genes (14) were amplified from TW-76005 genotype. The data gained from present study open up new ways to produce new varieties by breeding rust resistant germplasm to avoid the economic and food loss and varieties with improved characteristics.
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Keywords |
Wheat, germplasm, leaf rust, stem rust, stripe rust.
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Citation |
Haque et al.
Bioinformation 10(6): 371-377 (2014) |
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Edited by |
P Kangueane
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ISSN |
0973-2063
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Publisher |
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License |
This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License. |