In-Vitro and In-Silico characterization of Sophora interrupta plant extract as an anticancer activity



Pardhasaradhi Mathi1, Kumar Nikhil2, Nagavamsikrishna Ambatipudi3, Partha Roy|2, Venkata Raman Bokka4 & Mahendran Botlagunta1*



1Biomedical research Laboratory, Department of Biotechnology, K L University, Green fields, Vaddeswaram, Guntur 522 502, AndhraPradesh, India; 2Molecular Endocrinology Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247 667, Uttarakhand, India; 3Department of Biochemistry, Acharya Nagarjuna University, Guntur, India; 4Department of Basic Sciences-Chemistry, Madanapalle Institute of Technology and Science (MITS), Madanapalle 517 325, Chittoor District, Andhra Pradesh, India


Email; *Corresponding author


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Received March 05, 2014; Accepted March 06, 2014; Published March 19, 2014



Sophora interrupta belongs to the family of Fabaceae and the species in this genus have a diverse medicinal importance as a folk medicine for preventing many ailments including cancer. In order to evaluate the anticancer activity of S.interrupta, we have performed in vitro anti-oxidant, anti-inflammatory, anti-proliferative, and cell based anticancer activity in MCF-7 and PC-3 cell lines. Secondary metabolites of S.interrupta were used to identify anticancer compounds using Open Eye software. The antioxidant activity of the S.interrupta root ethylacetate (SEA) extract at 100 μg/ml is equal to that of ascorbic acid at 50 μg/ml. The anti-inflammatory activity of SEA is half of that of diclofenac at 50 μg/ml. Anticancer activity was detected by measuring the mitochondrial dehydrogenase activity (MTT assay). The half maximal inhibitory concentrations (IC50) for MCF-7 and PC-3 cell lines are 250 and 700 g/ml respectively. This was supported by the morphological changes such as membrane blebbing, cell detachment and rounded cell morphology when compared to the parental cells. In addition, we observed few green cells (live) over red cells (dead) based on the uptake of acridine orange and ethidium bromide dyes. Kaempferol-3-O-b-D-glucopyranoside, a Secondary metabolite of S.interrupta form 6 hydrogen bond interactions with Arg 202, Gln 207, Gly 227, Gly 229, Thr 231 and Ala 232 human DEAD box RNA helicase, DDX3 protein and is equivalent to crystal structure of adenosine mono phosphate to DDX3. Overall, it suggests that the SEA extract has anticancer compounds, and it can be used to enhance death receptor mediated cancer cell death.



Sophora interrupta, Apoptosis, cancer cell lines, DDX3.



Mathi  et al. Bioinformation 10(3): 144-151 (2014)

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P Kangueane






Biomedical Informatics



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