Genetic Variant of C-5312T Can Change Binding Pattern of Sp1 to Renin Enhancer that are Very Likely to Affect Renin Gene Expression

BACK TO CONTENTS | PDF | PREVIOUS | NEXT

Title	Genetic Variant of C-5312T Can Change Binding Pattern of Sp1 to Renin Enhancer that are Very Likely to Affect Renin Gene Expression
Authors	Mifetika Lukitasari¹, Jayarani F. Putri², Muladefi Choiriyah¹, Mohammad Saifur Rohman³* & Nashi Widodo²*
Affiliation	¹Nursing Department, Faculty of Medicine, University of Brawijaya, Malang, Indonesia; ²Biology Department, Faculty of Sciences, University of Brawijaya, Malang, Indonesia; ³Department of Cardiology and Vascular Medicine, Faculty of Medicine, Brawijaya University-Saiful Anwar General Hospital, Malang, Indonesia
Email	ippoenk@ub.ac.id; *Corresponding author
Article Type	Hypothesis
Date	Received December 10, 2013; Accepted December 15, 2013; Published December 27, 2013
Abstract	Renin distal enhancer plays a pivotal role in renin gene expression, and the genetic variants C-5312T of renin enhancer can affect renin gene transcription level. However, the mechanism associated with the transcription level changes remains unknown. Therefore, it is of interest to investigate the possible role of distal enhancer in regulating the expression of renin gene. Single nucleotide polymorphism in renin distal enhancer was identified in 34 hypertensive patients by automatic sequencing. The data showed that the renin enhancer from the patients have genetic variants C-5312T or C-5312T SNP. Hence, the functionality of the renin enhancer and influence of the genetic variants C-5312T on binding to Sp1 is studied. These results from the binding study suggested that Sp1 binds to the DNA in GC rich region. Thus, the genetic variant C-5312T has changed the binding pattern of Sp1 to renin enhancer. This is likely to influence Sp1 activity to stimulate the expression of renin gene. The binding of Sp1 to the cis-element will enhance transcription of renin gene. Thus, polymorphism within C-5312T might contribute to the reduction of renin transcription.
Citation	Lukitasari et al. Bioinformation 9(20): 1010-1012 (2013)
Edited by	P Kangueane
ISSN	0973-2063
Publisher	Biomedical Informatics
License	This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.