Characterization and phylogenetic analysis of antituberculous compound producing actinomycete strain D25 isolated from Thar Desert soil, Rajasthan

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Title	Characterization and phylogenetic analysis of antituberculous compound producing actinomycete strain D25 isolated from Thar Desert soil, Rajasthan
Authors	Manikkam Radhakrishnan¹, Venugopal Gopikrishnan², Arumugam Suresh¹, Nagamiah Selvakumar¹, Ramasamy Balagurunathan² & Vanaja kumar¹*
Affiliation	¹Department of Bacteriology, National Institute for Research in Tuberculosis, Chetpet, Chennai – 600031,Tamil Nadu, India; ²Department of Microbiology, Periyar University, Salem–600011, Tamil Nadu, India.
Email	vanaja_kumar51@yahoo.co.in; *Corresponding author
Article Type	Hypothesis
Date	Received October 09, 2012; Accepted October 15, 2012; Published January 09, 2013
Abstract	During the course of the anti-infective drug discovery programme, actinomycete strain D25 was recovered from the Thar Desert soil, Rajasthan, India. Actinomycin type of compound isolated from the strain D25 showed promising activity against multi drug resistant and extensively drug resistant M. tuberculosis isolates. The present study reports the characteristics and phylogenetic status of the actinomycete strain D25. Phenotypic and cell wall characteristics revealed that the strain belongs to the genus Streptomyces. Further 16s rRNA analysis confined the genus Streptomyces with 97% similarity to the closely related species Streptomyces althioticus KCTC 9752. The 16s rRNA sequence was submitted to GenBank with the accession number JN604533.1. According to Bossard et al. (2003) strain D25 was found to be a novel species of the genus Streptomyces from Thar Desert soil, Rajasthan.
Keywords	Streptomyces, 16s rRNA, phylogenetic analysis, Thar Desert, antituberculous compound
Citation	*Radhakrishnan et al.* Bioinformation 9(1): 018-022 (2013)
Edited by	P Kangueane
ISSN	0973-2063
Publisher	Biomedical Informatics
License	This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.