Comparative modeling of CCRL1, a key protein in masked immune diseases and virtual screening for finding inhibitor of this protein



Mohaddeseh Behjati1, Ibrahim Torktaz2, 4, Mehrdad Mohammadpour3, Gholamreza Ahmadian4*, Andrew J Easton5



1Isfahan University of Medical Sciences, Isfahan, Iran; 2Department of Biotechnology, Faculty of Advanced science and technologies, University of Isfahan, Hezarjarib St., 81746-73441, Isfahan, Iran; 3Department of Medicinal Chemistry, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, 81746-73461, Isfahan, Iran; 4Department of Molecular Genetics, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran; 5School of Life Sciences, University of Warwick, Coventry, UK.


Email; *Corresponding author


Article Type



Received February 22, 2012; Accepted March 08, 2012; Published April 13, 2012



Human CCRL1 belongs to the family of silent chemokine receptors. This transmembrane protein plays a role in blunting function of chemokines through binding to them. This will attenuate immune responses. Interaction between CCRL1 and CCL21 determines this immune extinction. Thus inhibiting the action of this atypical chemokine seems to stimulate immune responses especially in the case of suppressed and immune deficient conditions. In this study we predicted 3D structure of CCRL1 using comparative modeling and Hiddebn Markov Model algorithm. Final predicted model optimized by Modeller v9.8 and minimized regarding energy level using UCSF chimera candidate version1.5.3. ClasPro webserver was used to find interacting residues between CCRL1 and CCL21. Interacting residues were used as target for chemical inhibitors by simulated docking study. For finding potential inhibitors, library of KEGG compounds screened and 97 obtained chemicals docked against interacting residues between CCRL1-CCL21 and MolDock was used as docking scoring function. Results indicated that Hexadecanal is a potential inhibitor of CCRL1- CCL21 interaction. Inhibition of this interaction will increase intercellular level of CCl21 and interaction between CCL21 and CCR7 causes immune potentiaiton.



CCRL1, CCL21, CCR7, Docking, MolDock, Hexadecanal.



Behjati et al. Bioinformation 8(7): 336-340 (2012)

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P Kangueane






Biomedical Informatics



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