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Title |
In silico identification of miRNAs and their targets from the expressed sequence tags of Raphanus sativus |
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Authors |
Charuvaka Muvva1*, Lata Tewari2, K Aruna3, Pabbati Ranjit3, Zahoorullah S4, KA Matheen5, Hemanth Veeramachaneni6 |
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Affiliation |
1Muvva Biosolutions Pvt.Ltd, Bioinformatics Division, #301, No: 7-1-414/21, Kaushik Sai Rama Residency, Srinivasa colony (East) S.R.Nagar, Hyderabad-500038, India; 2Department of Bioscience and Biotechnology, Banasthali University, P.O.Banasthali Vidyapith, Rajasthan- 304022, India; 3Department of Biotechnology, JNTU College of Engineering, Pulivendula, Kadapa (Dist), Andhra Pradesh, India-516390; 4Department of Biotechnology, Joginpally B R Engineering College, Yenkapally, Moinabad, Ranga Reddy (Dist), Andhra Pradesh, India-500075; 5Department of Biotechnology, Chaitanya Bharathi Institute of Technology, Gandipet, Hyderabad-500075, India; 6Muvva Biosolutions Pvt.Ltd Bioinformatics Division, #301, No: 7-1-414/21, Kaushik Sai Rama Residency, Srinivasa colony (E), S.R.Nagar, Hyderabad-500038, India;
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charvakmuvva@gmail.com; *Corresponding author
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Article Type |
Hypothesis
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Date |
Received November 23, 2011; Accepted January 07, 2012; Published January 20, 2012
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Abstract |
MicroRNAs (miRNAs) are a novel growing family of endogenous, small, non- coding, single-stranded RNA molecules directly involved in regulating gene expression at the posttranscriptional level. High conservation of miRNAs in plant provides the foundation for identification of new miRNAs in other plant species through homology alignment. Here, previous known plant miRNAs were BLASTed against the Expressed Sequence Tag (EST) database of Raphanus sativus, and according to a series of filtering criteria, a total of 48 miRNAs belonging to 9 miRNA families were identified, and 16 potential target genes of them were subsequently predicted, most of which seemed to encode transcription factors or enzymes participating in regulation of development, growth and other physiological processes. Overall, our findings lay the foundation for further researches of miRNAs function in R.sativus.
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Keywords |
MicroRNA, in silico Identification, Raphanus sativus, expressed sequence tag, minimal free energy index, Radish
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Citation |
Muvva et al
Bioinformation 8(2): 098-103 (2012) |
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Edited by |
P Kangueane
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ISSN |
0973-2063
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Publisher |
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License |
This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License. |