Title

Analysis of simple sequence repeats (SSRs) dynamics in fungus Fusarium graminearum 

Authors

Rajender Singh*, Sonia Sheoran, Pradeep Sharma, Ravish Chatrath

Affiliation

Directorate of Wheat Research, Post Box 158, Karnal - 132001, India

Email

rajenderkhokhar@yahoo.com; *Corresponding author  

Article Type

Hypothesis

Date

Received July 26, 2010; Accepted January 06, 2011; Published February 15, 2011
 

The abundance and inherent potential for variations in simple sequence repeats (SSRs) or microsatellites resulted in valuable source for genetic markers in eukaryotes. We describe the organization and abundance of SSRs in fungus Fusarium graminearum (causative agent for Fusarium head blight or head scab of wheat. We identified 1705 SSRs of various nucleotide repeat motifs in the sequence database of F. graminearum. It is observed that mononucleotide repeats (62%) were most abundant followed by di- (20%) and trinucleotide repeats (14%). It is noted that tetra-, penta- and hexanucleotide repeats accounted for only 4% of SSRs. The estimated frequency of Class I SSRs (perfect repeats =20 nucleotides) was one SSR per 124.5 kb, whereas the frequency of Class II (perfect repeats >10 nucleotides and <20 nucleotides) was one SSR per 25.6 kb. The dynamics of SSRs will be a powerful tool for taxonomic, phylogenetic, genome mapping and population genetic studies as SSR based markers show high levels of allelic variation, codominant inheritance and ease of analysis. 

Keywords

SSR, microsatellites, Fusarium graminearum, EST
 

Citation

Singh et al. Bioinformation 5(10): 402-404 (2011)

Edited by

P Kangueane

ISSN

0973-2063

Publisher

Biomedical Informatics

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.