Title

Structure Prediction of Dihydroflavonol 4- Reductase and Anthocyanidin Synthase from Spinach 

Authors

Archna Sahay^{1, *} & Madhvi Shakya²

Affiliation

¹Department of Bioinformatics, MANIT, Bhopal- 462051, Madhya Pradesh, India; ²Department of Mathematics, MANIT, Bhopal- 462051, Madhya Pradesh, India

Email

sahayarchna@gmail.com

+91-0755-2670327-1622

+91-0755-2670562; * Corresponding author

Article Type

Hypothesis

Date

Received July 20, 2010; Accepted August 20, 2010; Published November 27, 2010
 

Spinach is an important dietary vegetable associated with beneficial health effects. Flavonoids have various biological activities such as antioxidant, antibacterial, and anticancer effect Flavonoid including anthocyanin provides brilliant and colored pigments in different plant tissues. Anthocyanidin synthase and dihydroflavonol 4-reductase are responsible for anthocyanin biosynthesis. They contributed in plant protection against UV-B radiation, microbial and herbivore pathogens. A 3D structures of anthocyanidin synthase and dihydroflavonol 4-reductase from spinach are constructed in this study through homology modeling. The homology modeling is done by using the MODELLER 9v7 software. The energy of models was minimized by applying molecular mechanics method. The root mean square deviation (RMSD) for C atoms between the template and the homology-modeled structures was estimated by CE program. The final models were assessed by PROCHECK and WHATCHECK which showed that the final refined models are reliable. 

Keywords

spinach; anthocyanidin synthase; dihydroflavonol 4-reductase; flavonoid, structure prediction.

Citation

Sahay & Shakya. Bioinformation, 5(6): 259-263, 2010

Edited by

P. Kangueane

ISSN

0973-2063

Publisher

Biomedical Informatics

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.